PMID: 8589468Oct 1, 1995Paper

Light microscopic visualization of plasma intrusion into microporous hollow fibers

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
B T Cheng, E F Leonard

Abstract

An immunohistochemical method has been developed to describe the pattern and extent of plasma intrusion into hydrophobic microporous membranes. Even though these membranes have very high calculated capillary intrusion pressures, they fill with plasma, sometimes during short-term use and routinely over the time spans characteristic of extracorporeal membrane oxygenation applications. It is not clear whether intrusion is widespread and caused by operating procedure or general properties of the membrane or localized and caused by defects in the membrane. The staining method reported here depends upon the fact that plasma proteins appear on pore walls only where the pores have been contacted by plasma. The selected plasma protein can be revealed, in sections viewed under the light microscope, by the immunospecific attachment of colloidal gold particles covered with an appropriate antibody. The particles are made visible by using them to nucleate precipitation of silver. Data are reported for Celanese polypropylene hollow fibers: 1) uniformly wetted with plasma; 2) locally intruded by centrifuging a plasma filled fiber covering a range of radii in a hematocrit centrifuge; and 3) locally intruded by wetting out the fiber with a "micr...Continue Reading

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
L W LundBrack G Hattler
Kyobu geka. The Japanese journal of thoracic surgery
S Teramoto
ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Katharina KindReiner Körfer
© 2021 Meta ULC. All rights reserved