PMID: 3761365Aug 1, 1986Paper

Light microscopical detection of single 5 and 20 nm gold particles used for immunolabelling of plasma membrane antigens with silver enhancement and reflection contrast

Journal of Microscopy
E C HoefsmitJ S Ploem

Abstract

To detect plasma membrane antigens, cytocentrifuge preparations of the macrophage-like cell line P388D1 were incubated with monoclonal antibodies and labelled with 5 and 20 nm gold particles conjugated to immunoglobulins or protein A. The 20 nm, but not the 5 nm, particles could be observed by reflection-contrast light microscopy. Single 5 nm particles, however, were clearly demonstrable with silver contrast enhancement, both in absorption contrast and in reflection contrast. The method proved to be compatible with cytochemical methods such as acid phosphatase.

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Citations

Jul 1, 1996·Histochemistry and Cell Biology·P M Lackie
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J Mole-BajerB R Brinkley
Mar 23, 2000·The Journal of Pathology·T J Filler, E T Peuker
Apr 1, 1989·Arthritis and Rheumatism·D M NordstromV B Reddy
May 1, 1988·Journal of Electron Microscopy Technique·A T Sumner
May 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J M Robinson, D D Vandré
Jun 27, 2019·Journal of Microscopy·Mayandi SivaguruMakoto Inoue

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