Light-Regulated Angiogenesis via a Phototriggerable VEGF Peptidomimetic.

Advanced Healthcare Materials
Roshna V NairAránzazu Del Campo

Abstract

The application of growth factor based therapies in regenerative medicine is limited by the high cost, fast degradation kinetics, and the multiple functions of these molecules in the cell, which requires regulated delivery to minimize side effects. Here a photoactivatable peptidomimetic of the vascular endothelial growth factor (VEGF) that allows the light-controlled presentation of angiogenic signals to endothelial cells embedded in hydrogel matrices is presented. A photoresponsive analog of the 15-mer peptidomimetic Ac-KLTWQELYQLKYKGI-NH2 (abbreviated P QK) is prepared by introducing a 3-(4,5-dimethoxy-2-nitrophenyl)-2-butyl (DMNPB) photoremovable protecting group at the Trp4 residue. This modification inhibits the angiogenic potential of the peptide temporally. Light exposure of P QK modified hydrogels provide instructive cues to embedded endothelial cells and promote angiogenesis at the illuminated sites of the 3D culture, with the possibility of spatial control. P QK modified photoresponsive biomaterials offer an attractive approach for the dosed delivery and spatial control of pro-angiogenic factors to support regulated vascular growth by just using light as an external trigger.

References

Apr 10, 2002·American Journal of Physiology. Cell Physiology·Gaoyuan CaoHorace M DeLisser
Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Luca Domenico D'AndreaCarlo Pedone
Jul 11, 2006·Angewandte Chemie·Günter Mayer, Alexander Heckel
Jan 4, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Laxminarayana KammariPetr Klán
Jan 19, 2007·Journal of Thrombosis and Haemostasis : JTH·E A Silva, D J Mooney
Mar 23, 2007·Organic & Biomolecular Chemistry·Douglas D Young, Alexander Deiters
Mar 19, 2008·Angewandte Chemie·Svea PetersenAranzazu del Campo
Jun 10, 2009·Journal of Translational Medicine·Gaetano SantulliGuido Iaccarino
Oct 16, 2009·Biomedical Microdevices·Bor-han ChuehShuichi Takayama
Feb 2, 2010·HFSP Journal·Alexandre SpechtMaurice Goeldner
Mar 19, 2011·Journal of the American Chemical Society·Verónica San MiguelAránzazu del Campo
May 28, 2011·Advanced Materials·Melanie WirknerAránzazu del Campo
Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Matthew J WebberSamuel I Stupp
Sep 20, 2011·Cell·Michael PotentePeter Carmeliet
Sep 23, 2011·Biomaterials·Karolina ChwalekCarsten Werner
Nov 8, 2011·Chembiochem : a European Journal of Chemical Biology·Melanie WirknerAránzazu del Campo
May 5, 2012·Biochemical Pharmacology·Federica FinettiLuca Domenico D'Andrea
Nov 10, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·April M KloxinKristi S Anseth
Oct 15, 2013·Nature Materials·Katarzyna A MosiewiczMatthias P Lutolf
Oct 24, 2013·Nature Materials·Daniel L Alge, Kristi S Anseth
Dec 18, 2013·Cytokine & Growth Factor Reviews·Prakash VempatiFeilim Mac Gabhann
Feb 5, 2014·Tissue Engineering. Part a·Widya MulyasasmitaSarah C Heilshorn
Jan 15, 2015·ACS Nano·Vivek A KumarJeffrey D Hartgerink
Feb 7, 2015·Advanced Materials·Chunyan BaoHe Tian
Mar 20, 2015·Drug Delivery and Translational Research·Edward A PhelpsAndrés J García
Aug 4, 2015·Frontiers in Bioengineering and Biotechnology·Amy H Van Hove, Danielle S W Benoit
Nov 22, 2011·Advanced Functional Materials·Tania R ChanS Michael Yu
Apr 25, 2016·Journal of Materials Science. Materials in Medicine·Yang YangYubo Fan
Mar 11, 2017·Biomaterials·Vladimíra MoulisováManuel Salmerón-Sánchez
Jul 8, 2017·Molecular BioSystems·Domenica CapassoLuca D D'Andrea
Aug 19, 2017·Chemical Society Reviews·Tobin E Brown, Kristi S Anseth
Apr 11, 2018·Chembiochem : a European Journal of Chemical Biology·Aleeza FarrukhAránzazu Del Campo

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