Light-regulated proteins in Limulus ventral photoreceptor cells

Visual Neuroscience
S C EdwardsB A Battelle

Abstract

The protein intermediates of the photoresponse and the modulation of this response in invertebrate photoreceptors are largely unknown. As a first step toward identifying these proteins, we have examined light-stimulated changes in protein phosphorylation in preparations of Limulus photoreceptors. Here we show that light modulates the level of phosphorylation of three proteins associated with Limulus ventral photoreceptors: the upper band of a 46-kD protein doublet (46A) and a 122-kD protein, which become more heavily phosphorylated in response to light, and the lower component of the 46-kD doublet (46B), which is phosphorylated in dark-adapted cells, but not in cells maintained in the light. In dark-adapted preparations, 46A is phosphorylated within 30 s after a flash of light and dephosphorylates over a period of many minutes. It is also a major substrate for calcium/calmodulin-dependent protein kinase (Wiebe et al., 1989); therefore, we speculate that 46A is involved in some aspect of dark adaptation. Interestingly, the level of phosphorylation of 46A is the same when measured from preparations maintained in complete darkness or ambient light for at least 1.5 h. The 122-kD phosphoprotein is the same protein which becomes phos...Continue Reading

References

Oct 1, 1978·The Journal of General Physiology·D S Bayer, R B Barlow
Nov 1, 1988·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·L KassR B Barlow
Jan 1, 1988·Visual Neuroscience·L Kass, G H Renninger
Aug 1, 1989·Visual Neuroscience·E M WiebeB A Battelle
Mar 1, 1989·The Journal of General Physiology·P M O'Day, M P Gray-Keller
Jun 11, 1982·Science·B A BatelleS C Chamberlain
Jan 1, 1984·Journal of Neurochemistry·B A Battelle, J A Evans
Jan 15, 1972·FEBS Letters·H Kühn, W J. Dreyer

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Citations

Aug 1, 1989·Visual Neuroscience·E M WiebeB A Battelle
Sep 6, 2002·Microscopy Research and Technique·Barbara-Anne Battelle
Mar 21, 2007·Biochemistry·Karen KemplerBarbara-Anne Battelle
Aug 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·B G CalmanB A Battelle

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