Light Regulation of delta-Aminolevulinic Acid Biosynthetic Enzymes and tRNA in Euglena gracilis.

Plant Physiology
S M Mayer, S I Beale

Abstract

Chlorophyll synthesis in Euglena, as in higher plants, occurs only in the light. The key chlorophyll precursor, delta-aminolevulinic acid (ALA), is formed in Euglena, as in plants, from glutamate in a reaction sequence catalyzed by three enzymes and requiring tRNA(Glu). ALA formation from glutamate occurs in extracts of light-grown Euglena cells, but activity is very low in dark-grown cell extracts. Cells grown in either red (650-700 nanometers) or blue (400-480 nanometers) light yielded in vitro activity, but neither red nor blue light alone induced activity as high as that induced by white light or red and blue light together, at equal total fluence rates. Levels of the individual enzymes and the required tRNA were measured in cell extracts of light- and dark-grown cells. tRNA capable of being charged with glutamate was approximately equally abundant in extracts of light- and dark-grown cells. tRNA capable of supporting ALA synthesis was approximately three times more abundant in extracts of light-grown cells than in dark-grown cell extracts. Total glutamyl-tRNA synthetase activity was nearly twice as high in extracts of light-grown cells as in dark-grown cell extracts. However, extracts of both light- and dark-grown cells we...Continue Reading

References

Feb 1, 1975·Analytical Biochemistry·U Fricke
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Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S I BealeV Dzelzkalns
Aug 1, 1970·Plant Physiology·K Nadler, S Granick

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Citations

Jun 1, 1995·Photosynthesis Research·Y J Avissar, P A Moberg
Mar 22, 2000·Plant Science : an International Journal of Experimental Plant Biology·G Drazic, M Bogdanovic
Apr 15, 1996·European Journal of Biochemistry·S Reinbothe, C Reinbothe

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