Light-triggered switching of liposome surface charge directs delivery of membrane impermeable payloads in vivo.

Nature Communications
Gabriela Arias-AlpizarFrederick Campbell

Abstract

Surface charge plays a fundamental role in determining the fate of a nanoparticle, and any encapsulated contents, in vivo. Herein, we describe, and visualise in real time, light-triggered switching of liposome surface charge, from neutral to cationic, in situ and in vivo (embryonic zebrafish). Prior to light activation, intravenously administered liposomes, composed of just two lipid reagents, freely circulate and successfully evade innate immune cells present in the fish. Upon in situ irradiation and surface charge switching, however, liposomes rapidly adsorb to, and are taken up by, endothelial cells and/or are phagocytosed by blood resident macrophages. Coupling complete external control of nanoparticle targeting together with the intracellular delivery of encapsulated (and membrane impermeable) cargos, these compositionally simple liposomes are proof that advanced nanoparticle function in vivo does not require increased design complexity but rather a thorough understanding of the fundamental nano-bio interactions involved.

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Citations

Mar 19, 2021·Nanoscale·Jinyu RongSijie Lin
May 22, 2021·Advanced Drug Delivery Reviews·Zehua LiuHélder A Santos
Jun 18, 2021·Advanced Drug Delivery Reviews·Xi HuDaishun Ling
Jul 3, 2021·Pharmaceutics·Christophe DeleheddeFederico Perche
Aug 19, 2020·ACS Nano·Hossein Mohammad-BeigiYuya Hayashi
Jun 22, 2021·Annual Review of Cell and Developmental Biology·Spencer Freeman, Sergio Grinstein

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Methods Mentioned

BETA
dynamic light scattering
transgenic
fluorescence imaging
size exclusion chromatography
Fluorescence
confocal microscopy

Software Mentioned

Leica Application Suite X
ImageJ
LabVIEW
Myocet
Cary WinUV

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