LightCycler-based differentiation of Mycobacterium abscessus and Mycobacterium chelonae

Journal of Clinical Microbiology
L SedlacekFranz-Christoph Bange

Abstract

In this study we introduce a rapid procedure to identify Mycobacterium abscessus (types I and II) and M. chelonae using LightCycler-based analysis of the hsp65 gene. Results from 36 clinical strains were compared with hsp65 gene restriction analysis and biochemical profiles of bacilli. As all three methods yielded identical results for each isolate, this procedure offers an excellent alternative to previously established nucleic acid amplification-based techniques for the diagnosis of mycobacterial diseases.

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Jul 5, 2003·Journal of Clinical Microbiology·Marion StermannFranz-Christoph Bange
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Citations

Jan 19, 2006·Clinical Microbiology Reviews·M J EspyT F Smith
May 27, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Björn Petrini
Jan 14, 2012·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Anna SajdudaJuan Carlos Palomino
Nov 8, 2013·Journal of Clinical Microbiology·Kai RügerFranz-Christoph Bange
Dec 21, 2007·The Korean journal of laboratory medicine·Seong-Ho KangEui Chong Kim

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