Lighting Up the Thioflavin T by Parallel-Stranded TG(GA) n DNA Homoduplexes

ACS Sensors
Jinbo ZhuErkang Wang

Abstract

Thioflavin T (ThT) was once regarded to be a specific fluorescent probe for the human telomeric G-quadruplex, but more other kinds of DNA were found that can also bind to ThT in recent years. Herein, we focus on G-rich parallel-stranded DNA and utilize fluorescence, absorbance, circular dichroism, and surface plasmon resonance spectroscopy to investigate its interaction with ThT. Pyrene label and molecular modeling are applied to unveil the binding mechanism. We find a new class of non-G-quadruplex G-rich parallel-stranded ( ps) DNA with the sequence of TG(GA) n can bind to ThT and increase the fluorescence with an enhancement ability superior to G-quadruplex. The optimal binding specificity for ThT is conferred by two parts. The first part is composed of two bases TG at the 5' end, which is a critical domain and plays an important role in the formation of the binding site for ThT. The second part is the rest alternative d(GA) bases, which forms the ps homoduplex and cooperates with the TG bases at the 5' end to bind the ThT.

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Citations

Jul 21, 2020·Methods and Applications in Fluorescence·Tulsi R DamasePeter B Allen
Feb 24, 2021·ACS Sensors·Lanqun Mao
Jun 13, 2021·Biochimie·Smita VermaNihar Ranjan
Jul 10, 2019·Journal of the American Chemical Society·Jinbo ZhuUlrich F Keyser
Mar 7, 2020·The Journal of Physical Chemistry Letters·Srikrishna PramanikSaptarshi Mukherjee
Apr 23, 2019·ACS Chemical Neuroscience·Donghee LeeYoungSoo Kim

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