Lignin Peroxidases, Manganese Peroxidases, and Other Ligninolytic Enzymes Produced by Phlebia radiata during Solid-State Fermentation of Wheat Straw.

Applied and Environmental Microbiology
T VaresA Hatakka

Abstract

The white rot fungus Phlebia radiata 79 (ATCC 64658) produces lignin peroxidase (LiP), manganese peroxidase (MnP), glyoxal oxidase (GLOX), and laccase in the commonly used glucose low-nitrogen liquid medium. However, the enzymes which this fungus utilizes for selective removal of lignin during degradation of different lignocellulosic substrates have not been studied before. Multiple forms of LiP, MnP, GLOX, and laccase were purified from P. radiata culture extracts obtained after solid-state fermentation of wheat straw. However, the patterns of extracellular lignin-modifying enzymes studied were different from those of the enzymes usually found in liquid cultures of P. radiata. Three LiP isoforms were purified. The major LiP isoform from solid-state cultivation was LiP2. LiP3, which has usually been described as the major isoenzyme in liquid cultures, was not expressed during straw fermentation. New MnP isoforms have been detected in addition to the previously reported MnPs. GLOX was secreted in rather high amounts simultaneously with LiP during the first 2 weeks of growth. GLOX purified from P. radiata showed multiple forms, with pIs ranging from 4.0 to 4.6 and with a molecular mass of ca. 68 kDa.

References

Sep 1, 1992·Applied and Environmental Microbiology·F S Archibald
May 15, 1990·Archives of Biochemistry and Biophysics·E KarhunenM L Niku-Paavola
Sep 15, 1988·The Biochemical Journal·M L Niku-PaavolaV Raunio
Jan 1, 1987·Annual Review of Microbiology·T K Kirk, R L Farrell
Dec 1, 1993·Applied and Environmental Microbiology·A B OrthM Tien
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P J Kersten
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·E SrebotnikK E Hammel

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Citations

Jan 14, 2005·Applied Microbiology and Biotechnology·René UllrichMartin Hofrichter
Jul 12, 2012·Applied Microbiology and Biotechnology·Christoph A GasserPhilippe F-X Corvini
May 9, 2006·The Journal of Eukaryotic Microbiology·Jie-Jie HaoPeng Zhang
Jun 8, 2011·Applied Biochemistry and Biotechnology·Chen Qi-HeSusanne Zibek
Sep 15, 2010·Pakistan Journal of Biological Sciences : PJBS·A R AhmadiJ Khanifar
Jan 3, 2018·Environmental Technology·Nazia KhatoonMuhammad Ishtiaq Ali
Nov 22, 1997·FEMS Microbiology Letters·A A LeontievskyA Hatakka
Feb 23, 2010·Journal of Basic Microbiology·Taina K LundellKristiina Hildén
Dec 18, 2007·Preparative Biochemistry & Biotechnology·Raziye Ozturk Urek, Nurdan Kasikara Pazarlioglu
Oct 13, 2005·Bioprocess and Biosystems Engineering·Akhila RajanT Emilia Abraham
Feb 14, 2006·FEMS Microbiology Reviews·Petr Baldrian
Sep 7, 2007·Applied Microbiology and Biotechnology·Kristiina HildénAnnele Hatakka
Sep 9, 2016·MicrobiologyOpen·Ayodeji O FaladeAnthony I Okoh
Feb 29, 2016·Food Science and Biotechnology·Tamires Carvalho Dos SantosMarcelo Franco
Jul 19, 2008·Applied Microbiology and Biotechnology·Henning BouwsHolger Zorn
Jul 6, 2014·Bioprocess and Biosystems Engineering·Ulla MoilanenTero Eerikäinen
Dec 26, 2001·Chemical Reviews·R ten Have, P J Teunissen
Oct 1, 1996·Applied and Environmental Microbiology·B W BoganD Cullen
Mar 2, 2006·Journal of Agricultural and Food Chemistry·Mohammad A KhiyamiWilliam J Kennedy
Jul 2, 2011·New Biotechnology·Pengfei XiaoRyuichiro Kondo

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