Ligninolytic enzyme production by Phanerochaete chrysosporium in plastic composite support biofilm stirred tank bioreactors

Journal of Agricultural and Food Chemistry
Mohammad A KhiyamiWilliam J Kennedy

Abstract

Phanerochaete chrysosporium (ATCC 24725) produced lignin peroxidase (LiP) and manganese peroxidase (MnP) in defined medium in plastic composite support (PCS) biofilm stirred tank reactors. Laccase was not detected. The formation of the Ph. chrysosporium biofilm on the PCS was essential for the production of MnP and LiP. The bioreactor was operated as a repeat batch, and no reinoculation was required between batches. Peroxidase production was influenced by 5 min purging of the bioreactor with pure oxygen or continuous aerating with a mixture of air and oxygen at a flow rate of 0.005 vvm. Continuous aeration and 300 rpm agitation with 3 mM veratryl alcohol addition on days 0 and 3 demonstrated the highest lignin peroxidase production on day 6 with means of 50.0 and 47.0 U/L. Addition of veratryl alcohol and MnSO(4) on day 0 with 300 rpm agitation and continuous aeration at 0.005 vvm (air flow rate in L/min divided by the reactor working volume in liters) hastens the production of MnP with final yield of 63.0 U/L after 3 days. Fourteen repeated batches fermentation were performed without contamination due to low pH (4.5) and aseptic techniques employed.

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Citations

Sep 24, 2008·Applied Microbiology and Biotechnology·Deepak Singh, Shulin Chen
May 4, 2010·Applied Microbiology and Biotechnology·Kuan-Chen ChengJeffrey M Catchmark
Jun 29, 2012·Molecular Biotechnology·Aydin BerenjianFariba Dehghani
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Aug 7, 2021·Frontiers in Bioengineering and Biotechnology·Chaoyong LiaoBingkun Zhang

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