Ligninolytic System Formation by Phanerochaete chrysosporium in Air.

Applied and Environmental Microbiology
N RothschildCarlos G Dosoretz

Abstract

This study characterizes the effect of oxygen concentration on the synthesis of ligninolytic enzymes by Phanerochaete chrysosporium immobilized on polyurethane foam cubes in a nonimmersed liquid culture system and maintained under different carbon-to-nitrogen (C/N) ratios and levels. Lignin peroxidase (LIP) activity was obtained in cultures exposed to air when the C/N ratio was low (7.47), i.e., when nitrogen levels were high (C/N = 56/45 mM) or carbon levels were low (C/N = 5.6/4.5 mM). At the low C/N ratio, the fungus was carbon starved and did not produce extracellular polysaccharides. At a high C/N ratio (153), i.e., under conditions of excess carbon (nitrogen limitation) (C/N = 56/2.2 mM), cultures exposed to air produced large amounts of polysaccharide, and LIP activity was detected only in cultures exposed to pure oxygen. Under high-nitrogen conditions, LIP production was 1,800 U/liter in cultures exposed to pure oxygen and 1,300 U/liter in cultures exposed to air, with H1 and H2 being the main isoenzymes. The oxygen level did not significantly alter the isoenzyme profile, nor did low-carbon conditions. The formation of manganese peroxidase was generally less affected by the oxygen level than that of LIP but was consider...Continue Reading

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Citations

Jul 9, 2008·Applied and Environmental Microbiology·Avi MatityahuPaula A Belinky
May 8, 2013·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Chang-Young HongIn-Gyu Choi
Dec 24, 2009·Canadian Journal of Microbiology·Paramjit Kaur Bajwa, Daljit Singh Arora
Jun 25, 2014·Enzyme Research·Buddolla ViswanathGolla Narasimha
Nov 7, 2003·Applied and Environmental Microbiology·Paula A BelinkyCarlos G Dosoretz
Nov 26, 2009·Folia Microbiologica·C NovotnýV Sasek
Nov 24, 1999·Archives of Biochemistry and Biophysics·N RothschildC Dosoretz
Mar 1, 1997·Applied and Environmental Microbiology·N RothschildC G Dosoretz

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