PMID: 9174983Mar 1, 1997Paper

Limitation of Hu-PBL-scid mouse model in direct application to immunotoxicity assessment

Journal of Pharmacological and Toxicological Methods
H M KimG T Oh

Abstract

Hu-PBL-scid mice were directly introduced to the methods of immunotoxicity assessments. Human IgG and IgM was detected 1 week after transplantation. Cyclosporin A (CsA) and cyclophosphamide (CP), which were injected i.p. 4 weeks after transplantation, decreased the serum concentration of IgM after 2-4 days of treatment but not that of IgG. Lymphocyte proliferation induced by various mitogens and primary T-dependent antibody responses to sheep red blood cells could not be measured by using splenocytes of hu-PBL-scid mice. These results were correlated with the fact that human cells were not detected in the spleen, thymus, or blood of hu-PBL-scid mouse but were detected in lymph nodes of the intestine, which were observed by flow cytometric and immunohistochemical examinations. The present results suggest using hu-PBL-scid mice in routine immunotoxicity investigations: lymph nodes of intestines could be used as the lymphocyte source. In addition, the determination of serum Ig concentration might be used as a experimental item.

References

Feb 1, 1992·The Journal of Experimental Medicine·M Tary-Lehmann, A Saxon
Dec 1, 1991·Immunological Reviews·B E TorbettD E Mosier
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A ChambersN Hozumi
Oct 1, 1991·Cancer Metastasis Reviews·B M Mueller, R A Reisfeld
Oct 1, 1990·The Journal of Experimental Medicine·R NamikawaJ M McCune
Jan 1, 1994·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·P L PollockM I Luster

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Citations

Feb 22, 2002·Parasite Immunology·Matthew D Taylor, Kathryn J Else

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