Limitations and advantages of MS-HRM and bisulfite sequencing for single locus methylation studies

Expert Review of Molecular Diagnostics
Tomasz K WojdaczLise L Hansen

Abstract

The methylation-sensitive high-resolution melting (MS-HRM) protocol, as described by Wojdacz and Dobrovic, enables detection of a methylated template in an unmethylated background, with sensitivity similar to that of methylation-specific PCR (MSP). Furthermore, MS-HRM-based methylation screening is cost, labor and time efficient in contrast to direct bisulfite sequencing, which, therefore, is unsuitable as a screening method, but is still required to reveal the methylation status of individual CpG sites. In some experiments, detailed information on the methylation status of individual CpGs may be of interest for at least a subset of samples from MS-HRM-based methylation screening. For those samples, sequencing-based methodology has to be coupled with the MS-HRM protocol to investigate the methylation status of single CpG sites within the locus of interest. In this article, we review the limitations and advantages of MS-HRM and bisulfite sequencing protocols for single-locus methylation studies. Furthermore, we provide the insights into interpretation of the results obtained when a combination of the protocols is used for single-locus methylation studies.

References

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Mar 11, 2010·Expert Review of Molecular Diagnostics·Jesse L MontgomeryCarl T Wittwer

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Citations

Apr 11, 2013·Expert Review of Molecular Diagnostics·Surbhi JainYing-Hsiu Su
Dec 8, 2015·Environmental and Molecular Mutagenesis·Huiqi LiMaria Albin
Jan 7, 2018·Biochemical Genetics·Diana Chávez-LizárragaErika Chavira-Suárez
Nov 30, 2016·Biology Open·Guadalupe S López-ÁlvarezSaúl Villa-Treviño
Nov 14, 2019·Chemical Society Reviews·Mostafa Kamal MasudYusuke Yamauchi
Nov 13, 2019·International Journal of Legal Medicine·Farzeen KaderAdemola O Olaniran
Aug 31, 2019·International Journal of Cancer. Journal International Du Cancer·Karolina PrajzendancAnna Jakubowska
Apr 3, 2012·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Joshua HihathNongjian Tao
Feb 7, 2019·Scientific Reports·Chetan BakshiVeena Dhawan
Feb 4, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Goot Heah KhorThong Kwai Lin
Oct 19, 2019·World Journal of Clinical Cases·Yu-Xia Zhan, Guang-Hua Luo
May 18, 2017·Experimental and Molecular Pathology·Iben DaugaardLise Lotte Hansen
Jul 12, 2021·Seminars in Perinatology·Robert C PeeryLanlan Shen

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