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Limitations and challenges of genetic barcode quantification

Scientific Reports

Mar 4, 2017

Lars ThieleckeKerstin Cornils

Abstract

Genetic barcodes are increasingly used to track individual cells and to quantitatively assess their clonal contributions over time. Although barcode quantification relies entirely on counting sequencing reads, detailed studies about the method's accuracy are still limited. We report on ...read more

Mentioned in this Paper

Study
HEK293 Cells
Nucleic Acid Amplification Tests
Nucleic Acid Sequencing
Gene Amplification Technique
Clone
Sequencing
Cell Density
Sequence Determinations, DNA
DNA Barcoding, Taxonomic
Paper Details
References
  • References38
  • Citations8
1234

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Limitations and challenges of genetic barcode quantification

Scientific Reports

Mar 4, 2017

Lars ThieleckeKerstin Cornils

PMID: 28256524

DOI: 10.1038/srep43249

Abstract

Genetic barcodes are increasingly used to track individual cells and to quantitatively assess their clonal contributions over time. Although barcode quantification relies entirely on counting sequencing reads, detailed studies about the method's accuracy are still limited. We report on ...read more

Mentioned in this Paper

Study
HEK293 Cells
Nucleic Acid Amplification Tests
Nucleic Acid Sequencing
Gene Amplification Technique
Clone
Sequencing
Cell Density
Sequence Determinations, DNA
DNA Barcoding, Taxonomic

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

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Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

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DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes

Summarizing specific profiles in Illumina sequencing from whole-genome amplified DNA(opens in new tab)

DNA Research : an International Journal for Rapid Publication of Reports on Genes and GenomesDecember 20, 2013
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Paper Details
References
  • References38
  • Citations8
1234

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