Limitations in the screening of potentially anti-cryptosporidial agents using laboratory rodents with gastric cryptosporidiosis

Folia Parasitologica
Andrea ValigurovaIvona Foitová

Abstract

The emergence of cryptosporidiosis, a zoonotic disease of the gastrointestinal and respiratory tract caused by Cryptosporidium Tyzzer, 1907, triggered numerous screening studies of various compounds for potential anti-cryptosporidial activity, the majority of which proved ineffective. Extracts of Indonesian plants, Piper betle and Diospyros sumatrana, were tested for potential anti-cryptosporidial activity using Mastomys coucha (Smith), experimentally inoculated with Cryptosporidium proliferans Kváč, Havrdová, Hlásková, Daňková, Kanděra, Ježková, Vítovec, Sak, Ortega, Xiao, Modrý, Chelladurai, Prantlová et McEvoy, 2016. None of the plant extracts tested showed significant activity against cryptosporidia; however, the results indicate that the following issues should be addressed in similar experimental studies. The monitoring of oocyst shedding during the entire experimental trial, supplemented with histological examination of affected gastric tissue at the time of treatment termination, revealed that similar studies are generally unreliable if evaluations of drug efficacy are based exclusively on oocyst shedding. Moreover, the reduction of oocyst shedding did not guarantee the eradication of cryptosporidia in treated individua...Continue Reading

References

May 1, 1987·The American Journal of Tropical Medicine and Hygiene·R T Kilani, L Sekla
Feb 1, 1986·The Journal of protozoology·W L Current, N C Reese
Feb 1, 1995·International Journal for Parasitology·P J O'Donoghue
Nov 1, 1995·The Journal of International Medical Research·G TatarG Hasçelik
Mar 1, 1997·European Journal of Gastroenterology & Hepatology·G VenturaM B Lucia
Apr 29, 1998·Advances in Parasitology·S Tzipori
Dec 5, 1998·Journal of Dairy Science·B C Anderson
Dec 13, 2000·International Journal for Parasitology·R FayerS J Upton
Mar 3, 2001·The Journal of Parasitology·D MooreJ A Harp
May 31, 2002·The New England Journal of Medicine·Xian-Ming ChenNicholas F LaRusso
Feb 15, 2003·International Immunopharmacology·Bilal Bin-HafeezS Raisuddin
Feb 19, 2004·Postgraduate Medical Journal·N Pickerd, D Tuthill
Sep 27, 2005·Advances in Parasitology·R C A ThompsonN S Hijjawi
Mar 21, 2006·Parasitology Research·S PerrucciG Flamini
Mar 21, 2007·Digestive Diseases and Sciences·David B RamsayMarie L Borum
Dec 12, 2007·The Journal of Eukaryotic Microbiology·Andrea ValigurováJirí Vávra
Dec 26, 2007·International Journal for Parasitology·Andrea ValigurováJan Slapeta
Sep 26, 2008·Parasite : Journal De La Société Française De Parasitologie·G Gargala
Jan 10, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ingrid LindhSören Andersson
Jun 24, 2009·Experimental Parasitology·Una Ryan
Jun 27, 2009·Parasitology Research·M ShahiduzzamanA Daugschies
May 11, 2011·Parasitology Research·Liang WuJian-ping Cao
Jan 9, 2013·Clinical Microbiology Reviews·Maha BouzidKevin M Tyler
Oct 17, 2013·Parasitology·Janka MelicherováAndrea Valigurová
Mar 19, 2014·Veterinary Parasitology·Julie PetermannChristophe Chartier
Sep 26, 2015·Plant Biotechnology Journal·Charlotta HanssonNils Y Lycke
Oct 9, 2015·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Alex Akira Nakamura, Marcelo Vasconcelos Meireles
Nov 21, 2017·Experimental Parasitology·Radka PeckováIvona Foitová
Dec 2, 2017·Scientific Reports·H C Morrogh-BernardM Olšanský
Mar 6, 2018·European Journal of Protistology·Martin KváčBohumil Sak
Mar 10, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Letizia RomeoEmanuela Mazzon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.