Limitations in transplantation of astroglia-biomatrix bridges to stimulate corticospinal axon regrowth across large spinal lesion gaps

Neuroscience Letters
Ronald DeumensElbert A J Joosten

Abstract

Regrowth of injured axons across rather small spinal cord lesion gaps and subsequent functional recovery has been obtained after many interventions. Long-distance regeneration of injured axons across clinically relevant large spinal lesion gaps is relatively unexplored. Here, we aimed at stimulating long-distance regrowth of the injured corticospinal (CS) tract. During development, an oriented framework of immature astrocytes is important for correct CS axon outgrowth. Furthermore, a continuous growth promoting substrate may be needed to maintain a CS axon regrowth response across relatively large spinal lesion gaps. Hence, we acutely transplanted poly(D,L)-lactide matrices, which after seeded with immature astrocytes render aligned astrocyte-biomatrix complexes (R. Deumens, et al. Alignment of glial cells stimulates directional neurite growth of CNS neurons in vitro. Neuroscience 125 (3) (2004) 591-604), into 2-mm long dorsal hemisection lesion gaps. In order to create a growth promoting continuum, astrocyte suspensions were also injected rostral and caudal to the lesion gap. During 2 months, locomotion was continuously monitored. Histological analysis showed that astrocytes injected into host spinal tissue survived, but did n...Continue Reading

References

Jul 1, 1992·Journal of Neurobiology·S M StrittmatterM C Fishman
Feb 1, 1995·Journal of Neurotrauma·D M BassoJ C Bresnahan
Jan 20, 1997·The Journal of Comparative Neurology·P G PopovichB T Stokes
Nov 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yona GoldshmitAnn M Turnley
Apr 29, 2005·Neuroreport·Adele WoodhouseMeng Inn Chuah
Jul 30, 2005·Experimental Neurology·Michelle L StarkeyElizabeth J Bradbury
Nov 8, 2005·Progress in Neurobiology·Ronald DeumensElbert A J Joosten

❮ Previous
Next ❯

Citations

Sep 22, 2012·Tissue Engineering. Part a·Peter S DonoghueSusan C Barnett
Jun 30, 2009·Neuroscience Letters·José D Carrillo-RuizLuis C Jiménez-Botello
Oct 2, 2007·Biomaterials·Annette SørensenSusan C Barnett
Jun 26, 2008·Journal of Neuroscience Methods·A BozkurtN Pallua
Jun 26, 2018·Journal of Materials Science. Materials in Medicine·Aleš HejčlPavla Jendelová
Jan 30, 2018·Frontiers in Cellular Neuroscience·Shengwen LiuRadhika Puttagunta

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes in Repair & Regeneration

Astrocytes are glial cells found within the CNS and are able to regenerate new neurons. They become activated during CNS injury and disease. The activation leads to the transcription of new genes and the repair and regeneration of neurons. Discover the latest research on astrocytes in repair and regeneration here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.