Limitations of the GENSAT Egr1-EGFP transgenic mouse strain for neural circuit activity mapping.

Neuroscience Letters
Aubrey M DemchukBruce L McNaughton

Abstract

Transgenic immediate-early gene reporter mouse strains are valuable tools for studying activity-dependent neural cell populations in vivo. However, routine characterization of the Gene Expression Nervous System Atlas (GENSAT) "Egr1-EGFP" reporter mouse strain produced results that were highly inconsistent with endogenous Egr1 expression. Activity-dependent EGFP expression was not observed, and EGFP protein did not co-localize with native Egr1 protein. This precautionary study outlines the limitations of the Egr1-EGFP transgenic line as a tool to study the activity-dependent expression of Egr1 and emphasizes the necessity of taking into account the potential loss of regulatory elements, stability determinants, or translational modulation in transgenic reporter strains.

Citations

Mar 7, 2021·International Journal of Molecular Sciences·Wing Yip TamKwok-Kuen Cheung

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