Limited accuracy of transurethral and periurethral intrasphincteric injections of cellular suspension

Neurourology and Urodynamics
Anna BurdzinskaLeszek Paczek

Abstract

The efficacy of cell therapy in patients with stress urinary incontinence (SUI) is lower than expected. The aim of this study was to determine the injection accuracy rate both with transurethral and periurethral route. Autologous intraurethral cell transplantation was performed in female goats. The cells were injected either periurethrally (PERI group, two depots/animal, n = 8) or transurethrally (TRANS group, eight depots/animal, n = 11). Transurethral injections were performed under endoscopic guidance. The number and distribution of cell depots in urethras were analyzed in the three-step protocol: 1) screening of whole explants by in vivo imaging system; 2) systematic microscopic analysis of raw 10 μm cross-sections; 3) immunohistochemistry. As control, four urethras collected 1 day after transurethral transplantation were used. Episodes of cell suspension leakages after needle withdrawal were noted. In all experimental animals depots were identified in the urethral wall 28 days after transplantation. The mean percentage of depots located in the urethral wall in relation to all performed injections amounted to 68.7% and 67.0% for PERI and TRANS groups, respectively. The mean proportions of depots which were identified in ext...Continue Reading

References

Aug 9, 2007·European Urology·Michael MitterbergerHannes Strasser
Jan 22, 2008·International Urogynecology Journal and Pelvic Floor Dysfunction·L K CarrM B Chancellor
Jan 13, 2012·Cell Transplantation·Daniel EberliAnthony Atala
Nov 13, 2012·The Journal of Urology·Alan J Wein
Mar 4, 2014·The Journal of Urology·Kenneth M PetersMichael B Chancellor
Nov 13, 2015·Expert Opinion on Biological Therapy·Marta PokrywczynskaTomasz Drewa
Nov 17, 2015·Central European Journal of Urology·Janusz Zajda, Fawzy Farag
Dec 4, 2015·Journal of Tissue Engineering and Regenerative Medicine·Anthony AtalaJames J Yoo
May 27, 2016·International Journal of Urology : Official Journal of the Japanese Urological Association·Bartosz DybowskiPiotr Radziszewski
Oct 16, 2016·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Anna BurdzinskaLeszek Paczek

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Citations

Sep 19, 2020·Cell & Bioscience·Yizhen Sima, Yisong Chen
Jul 3, 2021·International Journal of Molecular Sciences·Walter LinzenboldMarkus D Enderle

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