Limited heterogeneity of rearranged T cell receptor V alpha and V beta transcripts in synovial fluid T cells in early stages of rheumatoid arthritis

Arthritis and Rheumatism
D C FischerH D Haubeck

Abstract

The identification of activated T cells in synovial fluid and synovium, and the association of rheumatoid arthritis (RA) with specific HLA-DR restriction elements, strongly suggest that these T cells play a critical role in the etiology and pathogenesis of RA. Analysis of the T cell receptor (TCR) repertoire in the early stages of RA might be an approach to identify those T cells involved in the initiation and/or perpetuation of the disease. TCR V alpha and V beta transcripts of synovial T cells, sampled at the early stages of RA, were amplified by reverse transcriptase-polymerase chain reaction. HLA-DR subtyping was determined by serologic analysis and dot-blot hybridization of polymerase chain reaction amplification products using digoxigenin-labeled, sequence-specific oligonucleotide probes. Our findings showed a limited heterogeneity of V alpha and V beta TCRs in synovial fluid T cells, and a preferential usage of TCR V alpha 17 in early RA. In contrast, in the later stages of RA, a more polyclonal TCR V alpha and V beta gene usage was observed. Our results support the view that induction of RA is driven by an oligoclonal immune response to an unknown antigen. These findings also suggest a pathogenetic role for V alpha 17 T...Continue Reading

References

Aug 1, 1992·The Journal of Clinical Investigation·W V WilliamsD B Weiner
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y UematsuM Steinmetz
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M D HowellS W Brostoff
Jan 1, 1991·Annual Review of Immunology·I R Cohen
Jan 1, 1991·European Journal of Immunology·M SioudJ B Natvig
Jan 1, 1991·Annual Review of Immunology·G T Nepom, H Erlich
Jan 1, 1990·Scandinavian Journal of Immunology·A M MiltenburgF C Breedveld
Jun 1, 1990·Human Immunology·M K ChatilaJ T Kurnick
May 1, 1988·The Journal of Experimental Medicine·S S ZamvilH Acha-Orbea
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K SakaiL Steinman
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A D DubyD A Fox
Jan 1, 1989·Annual Review of Immunology·H Acha-OrbeaH O McDevitt
Apr 1, 1987·The Journal of Experimental Medicine·P ConcannonL E Hood
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I StamenkovicJ T Kurnick
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J M Leiden, J L Strominger
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A D Duby, J G Seidman
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P ConcannonL Hood
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M H KleinL Hood
Sep 1, 1986·The Journal of Experimental Medicine·N KimuraT W Mak
Nov 1, 1986·The Journal of Experimental Medicine·G SiuL E Hood
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A TunnacliffeT H Rabbitts
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K IkutaT Honjo
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y YanagiT W Mak
Jan 1, 1984·The Journal of Clinical Investigation·J HoloshitzI R Cohen
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert

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Citations

Jul 2, 1999·Springer Seminars in Immunopathology·P Moss, J Bell
Feb 26, 1999·International Reviews of Immunology·A CantagrelA Alam
Jun 9, 2001·Rheumatic Diseases Clinics of North America·L W Moreland
Jul 27, 2001·Clinical and Experimental Immunology·Y HashimotoT Kanematsu
Jan 24, 2018·Annals of the Rheumatic Diseases·Catriona T PrendergastRobert A Benson
Dec 28, 2018·Clinical and Experimental Immunology·P WehrR Thomas
Apr 12, 1997·Biochimica Et Biophysica Acta·R W KinneF Emmrich
Jul 11, 2021·International Reviews of Immunology·Ronghua SongJin-An Zhang

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