Jul 1, 1975

Limited hydrolysis of tRNA by phosphodiesterase

Hoppe-Seyler's Zeitschrift für physiologische Chemie
G R Philipps, T Chiemprasert

Abstract

Digestion of tRNA by electrophoretically pure phosphodiesterase is limited to a short sequence of nucleotides at the 3'-terminus. On the average, four percent of all nucleotides can be released from tRNA. The optimum Mg2 concentration is 10mM and the optimum pH 9.2. The mode of action is a random attack by the enzyme on the substrate. The terminal AMP is completely removed at 15 degrees C after short incubation; about 400 mol of AMP were removed per min by 1 mol of enzyme. The following CMP residues are released much more slowly; at 15 degrees C incompletely, and at 37 degrees C more or less completely in 1 h. In about 50% of the tRNA molecules, the fourth nucleotide could be removed in very long incubations or with very high enzyme concentrations.

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Mentioned in this Paper

Alkalescens-Dispar Group
Phosphoric diester hydrolase
MT-TA gene
Triplet Codon-amino Acid Adaptor Activity
Transfer RNA
5'-Adenylic Acid
3'-CMP
Nucleotides
Residual Limb Support System for Wheelchair, Any Type
Terminal (End Postition)

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