Limited interference at the early stage of infection between two recombinant novirhabdoviruses: viral hemorrhagic septicemia virus and infectious hematopoietic necrosis virus.

Journal of Virology
Stéphane BiacchesiMichel Brémont

Abstract

The genome sequence of a hypervirulent novirhabdovirus, viral hemorrhagic septicemia virus (VHSV) French strain 23-75, was determined. Compared to the genome of the prototype Fil3 strain, a number of substitutions, deletions, and insertions were observed. Following the establishment of a plasmid-based minigenome replication assay, recombinant VHSV (rVHSV) was successfully recovered. rVHSV exhibits wild-type-like growth properties in vitro as well as in vivo in rainbow trout. The dispensable role of NV for the novirhabdovirus replication was confirmed by generating rVHSV-DeltaNV, in which the NV gene was deleted. This deletion mutant was shown to be as debilitated as that previously described for infectious hematopoietic necrosis virus (IHNV), a distantly related novirhabdovirus (S. Biacchesi, M. I. Thoulouze, M. Bearzotti, Y. X. Yu, and M. Bremont, J. Virol. 74:11247-11253, 2000). Recombinant VHSV and IHNV expressing tdTomato and GFP(max) reporter genes, respectively, were generated, demonstrating the potential of these rhabdoviruses to serve as viral vectors. Interestingly, rIHNV-GFP(max) could be recovered using the replicative complex proteins of either virus, whereas rVHSV-Tomato could be recovered only by using its own rep...Continue Reading

References

Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Nov 1, 1983·Virology·P Whitaker-DowlingD K Wilcox
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·S P WhelanG T Wertz
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·N D LawsonJ K Rose
Aug 10, 1999·Journal of Virology·M BearzottiM Bremont
Jul 19, 2000·The Journal of General Virology·S BiacchesiM Brémont
Feb 28, 2002·Journal of Virology·Stéphane BiacchesiMichel Brémont
Sep 19, 2003·The Journal of General Virology·Elizabeth R ChareEdward C Holmes
Sep 27, 2003·Journal of Virology·Kirsten M SpannMichael N Teng
Mar 30, 2004·Journal of Virology·Maria-Isabel ThoulouzeMichel Brémont
Apr 24, 2004·The Journal of General Virology·Katja Einer-JensenNiels Lorenzen
Dec 9, 2004·Diseases of Aquatic Organisms·M SnowR S Raynard
Feb 26, 2005·Journal of Virology·Young-Min LeeCharles M Rice
Jul 22, 2005·The Journal of General Virology·Martin LudlowW Paul Duprex
Aug 19, 2005·Retrovirology·Micha NetheAntoinette C van der Kuyl
Feb 9, 2007·Journal of Virology·Donna M TscherneCharles M Rice
Sep 18, 2007·The Journal of General Virology·Claudia ClausTeryl K Frey
May 17, 2008·Journal of Fish Diseases·A I de las HerasS I Pérez-Prieto
Jan 23, 2010·Science·Virginie DoceulGeoffrey L Smith

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Citations

Jun 10, 2011·Journal of Virology·Arun Ammayappan, Vikram N Vakharia
Feb 15, 2011·Veterinary Research·Stéphane Biacchesi
Mar 10, 2016·Viruses·Ronan N RouxelMichel Brémont
Jan 17, 2015·Applied Microbiology and Biotechnology·Blanca ChinchillaEduardo Gomez-Casado
Nov 23, 2019·Virology Journal·Gianpiero ZamperinValentina Panzarin
Sep 29, 2020·Frontiers in Microbiology·Valentina PanzarinNiels J Olesen
Nov 17, 2020·Frontiers in Microbiology·Laury BaillonStéphane Biacchesi
Mar 16, 2021·Frontiers in Immunology·Mikolaj AdamekDieter Steinhagen

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