Limited nucleotide changes in the Rev response element (RRE) during HIV-1 infection alter overall Rev-RRE activity and Rev multimerization

Journal of Virology
Emily A SloanDavid Rekosh


HIV-1 Rev and the Rev response element (RRE) enable a critical step in the viral replication cycle by facilitating the nuclear export of intron-containing mRNAs, yet their activities have rarely been analyzed in natural infections. This study characterized their genetic and functional variation in a small cohort of HIV-infected individuals. Multiple Rev and RRE sequences were obtained using single-genome sequencing (SGS) of plasma samples collected within 6 months after seroconversion and at a later time. This allowed the identification of cognate sequences that were linked in vivo in the same viral genome and acted together as a functional unit. Phylogenetic analyses of these sequences indicated that 4/5 infections were founded by a single transmission event. Rev and RRE variants from each time point were subjected to functional analysis as both cognate pairs and as individual components. While a range of Rev-RRE activities were seen, the activity of cognate pairs from a single time point clustered to a discrete level, which was termed the set point. In 3/5 patients, this set point changed significantly over the time period studied. In all patients, RRE activity was more sensitive to sequence variation than Rev activity and ac...Continue Reading


Apr 1, 1973·Virology·F L Graham, A J van der Eb
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·M BrayMarie-Louise Hammarskjold
Aug 1, 1997·Methods : a Companion to Methods in Enzymology·K Wehrly, B Chesebro
Oct 6, 1998·Annual Review of Biochemistry·A D Frankel, J A Young
Jan 19, 1999·Annual Review of Microbiology·V W Pollard, Michael H Malim
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·Marylyn M AddoHIV Controller Study Collaboration
Feb 22, 2003·Immunity·Kevin R BobbittKathleen L Collins
Sep 13, 2003·Science·Shannon M HarperKevin H Gardner
Jun 25, 2004·Molecular Biology and Evolution·G AchazJ Wakeley
Jan 14, 2005·Journal of Medical Virology·Angsana PhuphuakratPrasert Auewarakul
Mar 4, 2005·Virus Genes·Angsana Phuphuakrat, Prasert Auewarakul
May 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Brandon F KeeleGeorge M Shaw
Sep 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michal LegiewiczStuart F J Le Grice
Jun 3, 2009·The Journal of Experimental Medicine·Nilu GoonetillekeAndrew J McMichael
Jun 3, 2009·The Journal of Experimental Medicine·Jesus F Salazar-GonzalezGeorge M Shaw
Oct 19, 2010·Nature Structural & Molecular Biology·Matthew D DaughertyAlan Frankel
Oct 27, 2010·BMC Bioinformatics·Elena E GiorgiTanmoy Bhattacharya
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lina JosefssonS Palmer


Mar 2, 2016·Wiley Interdisciplinary Reviews. RNA·Jason D FernandesAlan Frankel
Jun 16, 2015·Viruses·Jason W Rausch, Stuart F J Le Grice
Nov 12, 2013·Revue de pneumologie clinique·G PeretC Raherison-Semjen
Jan 8, 2020·Current HIV Research·Patrick E H JacksonMarie-Louise Hammarskjold
Jan 11, 2020·Journal of Molecular Recognition : JMR·Nicole G RaadColin A Smith
May 6, 2016·AIDS Research and Human Retroviruses·Patrick E H JacksonMarie-Louise Hammarskjold
Mar 28, 2019·Scientific Reports·Bhargavi JayaramanAlan Frankel
Jun 1, 2014·The Journal of Biological Chemistry·Xiaolei ZhuangPaul T Wingfield
Apr 1, 2020·Current HIV Research·Chringma SherpaStuart F J Le Grice
Jan 17, 2020·Viruses·Chringma Sherpa, Stuart F J Le Grice
May 14, 2021·Molecular Therapy. Methods & Clinical Development·Nathan M JohnsonStephen E Braun

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