Limited Potential or Unfavorable Manipulations? Strategies Toward Efficient Mesenchymal Stem/Stromal Cell Applications

Frontiers in Cell and Developmental Biology
Antonina LavrentievaCornelia Lee-Thedieck

Abstract

Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. One of the reasons of such outcomes is non-standardized and often disadvantageous ex vivo manipulation of MSCs prior therapy. In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. The role of po...Continue Reading

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Citations

Aug 18, 2020·Langenbeck's Archives of Surgery·Stefan ReischlPhilipp-Alexander Neumann
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Marline KirschAntonina Lavrentieva
Feb 23, 2021·Frontiers in Bioengineering and Biotechnology·Carola SchmitzAntonina Lavrentieva
Apr 13, 2021·The Journal of Bone and Joint Surgery. American Volume·Venkata P MantripragadaGeorge F Muschler

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
FCS
RNAseq

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