PMID: 7371634Feb 1, 1980Paper

Limited proteolysis and a reactive cysteine residue define accesible regions in the native conformation of the adenovirus hexon protein

European Journal of Biochemistry
H Jörnvall, L Philipson

Abstract

The sulfhydryl group of one cysteine residue in the adenovirus hexon protein is accessible to alkylation in the absence of denaturating agents. This residue was [14C]carboxymethylated and characterized in peptides after proteolytic treatments. It was identified in two different types of digest and corresponded to the cysteine residue most accessible to oxidation. It is located close to residue 670 in the tentative amino acid sequence of the entire polypeptide. Trypsin has a highly limited action on the native hexon protein. Only three peptide bonds are fully susceptible to cleavage. These were found to occur in the N-terminal third of the protein, and were characterized as cleavages close to positions 150, 180 and 320 in the tentative structure of the polypeptide. Trypsin therefore liberates four fragments in major yield, with approximate Mr of 3000, 15000, 17000 and 78000. The smallest fragment corresponds to a highly acidic region. A limited, but less restricted susceptibility of hexon to chymotryptic cleavage was also found, and four major sites were characterized. The cysteine labelling and the proteolytic treatments define accessible regions in the native conformation of hexon. Together with previously assigned residue dis...Continue Reading

References

Feb 1, 1978·Analytical Biochemistry·G E TarrD J McKean
Mar 1, 1978·Analytical Biochemistry·D G KlapperJ D Capra
Feb 1, 1977·Analytical Biochemistry·C L ZimmermanJ J Pisano
Aug 1, 1976·Journal of Virology·B EdvardssonL Philipson
Oct 15, 1974·Journal of Molecular Biology·M Grütter, R M Franklin
Oct 1, 1974·European Journal of Biochemistry·H JornvallL Philipson
Jan 23, 1974·Biochemical and Biophysical Research Communications·H JörnvallL Philipson
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H PerssonJ Zabielski

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Citations

Jan 1, 1985·The International Journal of Biochemistry·J L MillánH Jörnvall
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H PerssonJ Zabielski
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H JörnvallE Appella
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A NygrenH Wigzell
Jan 1, 1980·European Journal of Biochemistry·N KalkkinenL Kääriäinen
Nov 1, 1980·European Journal of Biochemistry·A NilssonV Mutt
Jul 15, 1983·European Journal of Biochemistry·H von Bahr-LindströmH Jörnvall
Apr 2, 1984·European Journal of Biochemistry·J JefferyH Jörnvall
May 15, 1984·European Journal of Biochemistry·H von Bahr-LindströmH Jörnvall
Dec 17, 1984·European Journal of Biochemistry·J HempelH Jörnvall
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·J J Rux, R M Burnett
Oct 30, 1984·Biochemical and Biophysical Research Communications·M Ingelman-Sundberg, H Jörnvall
Jul 4, 2012·Viruses·Carmen San Martín
Sep 3, 1984·FEBS Letters·K TatemotoV Mutt

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