PMID: 8594359Jan 1, 1995Paper

Limited proteolysis of DNA polymerases as probe of functional domains

Methods in Enzymology
W H Konigsberg

Abstract

Various proteolytic enzymes have been used to probe for domains in DNA polymerases. Results with several DNA polymerases that have been subjected to partial proteolysis demonstrated that there is a modular organization with different activities located in separate domains. In the case of the Klenow fragment, these domains appear to be independent of each other. With other DNA polymerases, the question of modular independence is not settled. Limited proteolysis for probing structure has been used with many other proteins in addition to DNA polymerases and the information obtained has been helpful in interpreting function-structure relationships. It is a general approach and can be applied in situations where the existence of domains is suspected. The simplicity of the method and the ease of monitoring the outcome is probably the main reason for its widespread and increasing use in enzymology.

Citations

Dec 1, 1996·Protein Science : a Publication of the Protein Society·M Zhong, C Wu
Nov 25, 2003·Cell Stress & Chaperones·Gregory M NelsonDavid F Smith
Feb 13, 2004·The Journal of Biological Chemistry·Patricia E CarriganDavid F Smith
May 23, 2006·The Journal of Biological Chemistry·Brian R KepplerMichael B Jarstfer
Dec 16, 1998·The Journal of Biological Chemistry·B J ReardonM Sander
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Eric S MillerWolfgang Rüger
Sep 28, 1999·Journal of Bacteriology·J A Surtees, B E Funnell

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