LIMK2 acts as an oncogene in bladder cancer and its functional SNP in the microRNA-135a binding site affects bladder cancer risk

International Journal of Cancer. Journal International Du Cancer
Wei WangMin Ying

Abstract

LIM kinases modulate multiple aspects of cancer development, including cell proliferation and survival. As the mechanisms of LIMK-associated tumorigenesis are still unclear, we analyzed the tumorigenic functions of LIM kinase 2 (LIMK2) in human bladder cancer (BC) and explored whether the newly identified LIMK2 3´-UTR SNP rs2073859 (G-to-A allele) is correlated with clinical features. Expression levels of LIMK2 in 38 human BC tissues and eight cell lines were examined using quantitative real-time PCR and immunohistochemistry. LIMK2 was overexpressed in most BC tissues (27/38, 71%) and BC-derived cell lines (6/8), and was more frequently overexpessed in high-grade than low-grade BC (80% vs. 47%). The effects of LIMK2 on BC cell proliferation, survival and migration, were studied by overexpression and RNA interference approaches in vitro and in vivo. LIMK2 overexpression promoted proliferation, migration and invasion of BC cells, while LIMK2 depletion inhibited cell invasion and viability and induced growth arrest in vitro and in vivo. PCR-Restriction Fragment Length Polymorphism (RFLP) was used to genotype LIMK2 SNP rs2073859 and multivariate logistic regression applied to assess the relationship between allele frequency and cli...Continue Reading

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Citations

Apr 29, 2019·Archives of Pharmacal Research·Mee-Hyun LeeJung-Hyun Shim
Jun 3, 2021·Cancers·Moloud A SooreshjaniKavita Shah
Aug 14, 2021·Acta Pharmaceutica Sinica. B·Qingfeng YuGuohua Zeng

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Methods Mentioned

BETA
biopsy
surgical resection
PCR
Protein Assay
transfection
genotyping
Assay
flow cytometry
xenografts
xenograft

Software Mentioned

RNAhybrid

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