PMID: 7012435Mar 2, 1981

Limulus amebocyte lysate test for endotoxemia: investigations with a femtogram sensitive spectrophotometric assay

Klinische Wochenschrift
P C FinkJ Kozak

Abstract

The question of specificity of Limulus amebocyte lysate (LAL) test in the diagnosis of endotoxemia has been a limiting factor of its clinical application. Using a femtogram-sensitive spectrophotometric LAL assay 35 of 36 septic postoperative patients showed an excellent correlation (almost 100%) between positive LAL tests and culture-proven gram-negative bacteremia. Twenty patients of this group demonstrated a significant correlation (p < 0.05) between elevated total white blood cell counts and Escherichia coli Endotoxin equivalents (EcEe). All 22 liver cirrhotic patients with potentially enteric endotoxemia yielded LAL positive reactions (100%) but 9 of 22 correlated significantly as to leucocytosis and elevated EcEe (2 p < 0.01). A significant correlation between EcEe and leucocyte counts was found for all neonates and all parturients respectively (2 p < 0.001), 2 p < 0.01). In vitro tests showed that leucocytes gave positive LAL tests. Supernates of a Ficoll sedimented crude leucocyte preparations, induced LAL positivity in a dose dependent manner, while all reagents per se used in the preparation, were negative. This study suggests that heat-labile factors from leucocytes apart from endotoxin are responsible for positive LA...Continue Reading

References

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Citations

Oct 1, 1995·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·T KomatsubaraS Saito
Apr 16, 1998·Gut·W D ClementsB J Rowlands
Jan 7, 2016·Critical Reviews in Clinical Laboratory Sciences·Anne GnauckMarlena Cathorina Kruger
May 30, 2015·Journal of Lipid Research·Jean-Paul Pais de BarrosLaurent Lagrost
Mar 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A B LumsdenM H Kutner
Nov 29, 2008·Child Development·Irene M LoeHeidi M Feldman
Jun 25, 2010·Child Development·Nermeen E El NokaliElizabeth Votruba-Drzal
Jun 25, 2010·Child Development·Deborah Lowe VandellNICHD Early Child Care Research Network
Feb 2, 2018·Prevention Science : the Official Journal of the Society for Prevention Research·Trang Quynh NguyenRenee M Johnson
May 22, 2019·Journal of Speech, Language, and Hearing Research : JSLHR·Johanna M RudolphSimone Crotteau
Sep 14, 1999·The American Journal of Gastroenterology·C V SoongB J Rowlands

Related Concepts

Endotoxins
Alkalescens-Dispar Group
Differential White Blood Cell Count Procedure
Pleocytosis
Limulus Test
Spectrophotometry
Surgical Wound Infection
Pyemia

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