PMID: 1099258Sep 29, 1975

Limulus lysate test for gram-negative bacterial meningitis. Bedside application

JAMA : the Journal of the American Medical Association
S RossW Khan

Abstract

The limulus lysate on cerebrospinal fluid was evaluated in 335 infants and children as a method for the rapid diagnosis of Gram-negative bacterial meningitis. Positive limulus tests were obtained within one hour in 33 of 34 cases of Hemophilus influenzae meningitis; four additional patients with Gram-negative meningitis also showed positive limulus lysate tests. Conversely, 13 patients with Gram-positive bacterial meningitis all yielded negative limulus assays. All 48 cases of aseptic meningitis and 236 children with no meningitis showed negative limulus assays. Antibiotic therapy prior to hospitalization did not vitiate the validity of the test. A bedside adaptation of the limulus test, performed by house officers and medical students, showed approximately 98% agreement with the laboratory assay.

Citations

Apr 1, 1978·Journal of Clinical Pathology·P J Coloe
Apr 1, 1982·The Journal of Clinical Investigation·H ThadepalliA K Mandal
Jan 1, 1976·The New England Journal of Medicine·J FineR Hutton
Jan 25, 2012·Environmental Monitoring and Assessment·Abdulkareem AbdulraheemMuhammed Shahjahan
Sep 1, 1978·CRC Critical Reviews in Microbiology·M Solotorovsky, M Lynn
Jan 1, 1981·Critical Reviews in Clinical Laboratory Sciences·H D Riley
Jan 1, 1981·Critical Reviews in Clinical Laboratory Sciences·H D Riley
Dec 1, 1977·Current Problems in Pediatrics·R L Wientzen, G H McCracken
Sep 1, 1985·Journal of Clinical Periodontology·D H FineI D Mandel
Mar 1, 1981·The Journal of Infection·J KlasterskyJ Brihaye
Oct 26, 1978·The New England Journal of Medicine·D F Ransohoff, A R Feinstein

Related Concepts

Arachnida (invertebrate)
Bacteriological Techniques
Complete Blood Count
Cerebrospinal Fluid
Differential Diagnosis
Endotoxins
Lymphoid Cells
Pachymeningitis
Meningitis, Haemophilus Parainfluenzae
Meningitis, Pneumococcal, Penicillin-Resistant

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