Lin28A promotes IRF6-regulated aerobic glycolysis in glioma cells by stabilizing SNHG14.

Cell Death & Disease
Jinjing LuYixue Xue

Abstract

Warburg effect is a hallmark of cancer cells, wherein glycolysis is preferred over oxidative phosphorylation even in aerobic conditions. Reprogramming of glycometabolism is especially crucial for malignancy in glioma. RNA-binding proteins and long noncoding RNAs are important for aerobic glycolysis during malignant transformation. Thus, we determined the expression and function of RNA-binding protein Lin28A, long noncoding RNA SNHG14, and transcription factor IRF6 in human glioma cells to elucidate the mechanism(s) underlying their role in glycolysis. Quantitative real-time polymerase chain reaction and western blotting showed that Lin28A and SNHG14 were overexpressed and IRF6 was downregulated in glioma. Depleting Lin28A from cells decreased the stability and expression of SNHG14. Furthermore, depleting SNHG14 reduced IRF6 mRNA degradation by targeting its 3' untranslated region and inhibiting STAU1-mediated degradation, thereby increasing the expression of IRF6. PKM2 is an important enzyme in aerobic glycolysis, and GLUT1 is the primary transporter that facilitates glucose uptake. IRF6 inhibited the transcription of PKM2 and GLUT1, thereby impairing glycolysis and cell proliferation and inducing apoptosis in glioma. Notably, ...Continue Reading

References

Feb 24, 1956·Science·O WARBURG
Nov 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ralph J DeBerardinisCraig B Thompson
Jul 8, 2008·Cell Cycle·Caleb M Bailey, Mary J C Hendrix
Sep 6, 2008·Science·D Williams ParsonsKenneth W Kinzler
Feb 17, 2010·The International Journal of Biochemistry & Cell Biology·Sybille Mazurek
Jan 19, 2011·The Journal of Experimental Medicine·Amparo WolfAbhijit Guha
Feb 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Qian SunHongbing Zhang
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Elisabetta BottiAntonio Costanzo
Jun 5, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Amy Haseley ThorneBalveen Kaur
Oct 11, 2014·Nature Communications·Xiaoyu MaHuafeng Zhang
May 28, 2016·Stem Cell Research & Therapy·Craig K DochertyJohn R Mercer
Aug 1, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hongbao GuoYue Zhong
Dec 23, 2016·Nature Communications·Nkerorema Djodji DamasAnders H Lund
Sep 10, 2017·EMBO Reports·Xiang-Song WuYing-Bin Liu

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Citations

Nov 1, 2020·Cells·Christian T StackhouseChristopher D Willey
May 29, 2021·Clinical and Translational Medicine·Weiwei DongYunhui Liu
Jul 6, 2021·BioMed Research International·Xiao BingxiangDing Chao
Jun 29, 2021·Frontiers in Oncology·Jie QinXiangqi Meng
Sep 2, 2021·Cancer Control : Journal of the Moffitt Cancer Center·Huiqin GuoZhenjian Zhuo
Feb 7, 2022·Journal of Translational Medicine·Jingshan LiangAimin Li

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Methods Mentioned

BETA
surgical resection
PCR
transfection
Assay
flow cytometry
immunoprecipitation
RNA
RIP
pulldown
Pull-Down

Software Mentioned

DBTSS
SPSS

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