LIN28B/let-7 axis mediates pulmonary inflammatory response induced by diesel exhaust particle exposure in mice

Toxicology Letters
Guanglei WangYuxin Zheng

Abstract

Exposure to diesel exhaust particle (DEP) is closely related to inflammatory response in respiratory system. To understand the underlying molecular mechanism by which DEP induces pulmonary inflammatory response, we conducted DEP exposure experiments in vivo and in vitro. In vivo, each mouse was exposed to DEP suspension (100 μg of DEP) or vehicle only once in single intra-tracheal instillation (IT) section, or was exposed to DEP suspension (12.5 μg or 50 μg of DEP) or vehicle 12 times in repeated IT section. DEP exposure induced significant pathological injuries with substantial neutrophils infiltration and the increased level of pro-inflammatory cytokine IL-6 in mouse lungs. Consistently, elevated IL6 mRNA level was also observed in DEP treatment group (100 μg/ml) in vitro. In addition, DEP exposure exerted the similar influence on the expression of let-7d and let-7g microRNAs in vivo and in vitro. To verify the possible role of LIN28B/let-7 axis in the regulation of IL6 expression following DEP exposure, we applied RNAi technology in vitro, and found increased IL6 mRNA expression was alleviated or neutralized in DEP exposure groups after LIN28B silencing or after let-7d or let-7g over-expression. Taken together, we conclude t...Continue Reading

Citations

Oct 11, 2020·European Journal of Clinical Investigation·Tingting DiTao Bian
Nov 1, 2020·Allergy·Julie WeidnerMadeleine Rådinger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.