Abstract
Atherosclerosis and inflammation are more common in patients with diabetes than in patients without diabetes, and atherosclerosis progression contributes to inflammation. Therefore, anti-inflammatory therapy is important for the prognosis of patients with diabetes. Linagliptin is the only bile-excreted, anti-diabetic oral dipeptidyl peptidase-4 (DPP-4) inhibitor. Although the anti-inflammatory effects of DPP-4 inhibitors in vivo and in vitro have been reported, few in vitro studies have examined the effects of linagliptin using monocytes, which play a central role in arteriosclerosis-related inflammation. Herein, we assessed the anti-inflammatory effects of linagliptin in human U937 monocytes. U937 cells at densities of 1 × 106 cells/mL were cultured in Roswell Park Memorial Institute medium supplied with 10% fetal bovine serum and treated with 100 nM phorbol myristate acetate for 48 h for differentiation into macrophages. The media were replaced, and the cells were pretreated with 1, 5, 10, 50, and 100 nM linagliptin for 1 h or were left untreated. The media were then replaced again, and the cells were treated with 1 μg/mL lipopolysaccharide (LPS) or 10 nM interleukin (IL)-1β only, in combination with 1, 5, 10, 50, and 100 nM ...Continue Reading
References
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J L GoldsteinM S Brown
Dec 1, 1991·The Journal of Clinical Investigation·J L Witztum, D Steinberg
Jan 1, 1983·Annual Review of Biochemistry·M S Brown, J L Goldstein
Apr 29, 1993·Nature·R Ross
Sep 25, 2008·Journal of Clinical Pharmacology·S HüttnerK A Dugi
Jan 21, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Stefan BlechKlaus Wagner
Sep 17, 2010·Atherosclerosis·Nga N TaYan Huang
Jan 29, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuhiko YamamotoHiroshi Yamamoto
May 12, 2011·Journal of Cardiovascular Pharmacology·Nga N TaYan Huang
Jan 12, 2012·Cardiovascular Diabetology·Odd Erik JohansenHans-Juergen Woerle
Apr 24, 2012·Lancet·Nigel UnwinClara Weil
Jun 30, 2012·The Journal of Clinical Endocrinology and Metabolism·Antoine MakdissiParesh Dandona
Jun 15, 2014·Biochimica Et Biophysica Acta·Nina WronkowitzJürgen Eckel
May 23, 2015·Therapeutics and Clinical Risk Management·Baptist Gallwitz
Dec 17, 2016·Japanese Clinical Medicine·Yuya NakamuraYuji Kiuchi
Nov 9, 2017·Biochemical and Biophysical Research Communications·Munenori HiromuraTsutomu Hirano
Citations
Sep 15, 2020·BioMed Research International·Jiawei QiItaru Mizoguchi
Aug 23, 2019·International Journal of Molecular Sciences·Michał WicińskiBartosz Malinowski
Jun 15, 2019·Journal of Molecular Neuroscience : MN·Mohsen SedighiMehrdad Roghani
Jan 1, 2021·PloS One·Ming-Han HsiehJung-Ta Kao
Jul 3, 2021·International Journal of Molecular Sciences·Hideki KitauraItaru Mizoguchi
Oct 17, 2020·Drug Design, Development and Therapy·Haoran WangWei Shang