LINC00346 Acts as a Competing Endogenous RNA Regulating Development of Hepatocellular Carcinoma via Modulating CDK1/CCNB1 Axis

Frontiers in Bioengineering and Biotechnology
Jinglan JinFeng Wei

Abstract

Hepatocellular carcinoma (HCC) is one of the important types of liver cancer. LncRNA is an important regulatory factor that regulates many biological processes such as tumor cells during tumorigenesis and metastasis. LINC00346 has been associated with various types of liver cancer, but its role and regulatory mechanism in HCC remain unclear. In our study, we found the LINC00356-miR-199a-3p-CDK1/CCNB1 axis through bioinformatics analysis. The expressions of LINC00356, miR-199a-3p, CDK1, and CCNB1 in HCC and normal hepatocytes were determined by qRT-PCR and WB. The results showed that LINC00356, CDK1 and CCNB1 were highly expressed in HCC, while miR-199a-3p was lowly expressed. Dual luciferase reporter gene assay, RIP and RNA-pull down assays demonstrated the targeted binding relationship of LINC00346-miR-199a-3p-CDK1/CCNB1. Overexpressing LINC00460 and silencing miR-199a-3p promoted cell invasion, inhibited apoptosis of HCC, and arrested the cell cycle in S phase while opposite results were obtained when silencing LINC00346, CDK1, and CCNB1. LINC00346 indirectly affects liver cancer by promoting the expression of CDK1/CCNB1 through competitive adsorption of miR-199a-3p. In addition, the study also demonstrated that overexpressio...Continue Reading

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Citations

Jan 12, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Rongjie ZhangZexiong Chen
Apr 27, 2021·Experimental Biology and Medicine·Yiting QiaoJianxiang Chen

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Datasets Mentioned

BETA
GSE62232
GSE74618
GES62232

Methods Mentioned

BETA
protein assay
transfection
RIP
PCR
pull-down

Software Mentioned

pathview
TargetScan
Image Lab
clusterprofiler
SPSS
limma
STRING
cytocape

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