LINC00667/miR-449b-5p/YY1 axis promotes cell proliferation and migration in colorectal cancer

Cancer Cell International
Juan YuGuangsen Han

Abstract

Long non-coding RNAs (lncRNAs) have been defined as vital regulators in the progression of human cancers, including colorectal cancer (CRC). Long intergenic non-protein coding RNA 667 (LINC00667) is a tumor promoter in several cancer types, while its role in CRC remains to be unmasked. This study focused on exploring the potential function and regulatory mechanism of LINC00667 in CRC. qRT-PCR analysis was applied to detect the expression of LINC00667 in CRC cells. Loss-of function assays revealed the role of LINC00667 silencing in regulating CRC cell proliferation, apoptosis and migration. In vivo study demonstrated the effect of LINC00667 silencing on CRC cell growth. Mechanism experiments were conducted to determine the upstream or the downstream molecular mechanism of LINC00667 in CRC cells. LINC00667 was expressed at high level in CRC cells. LINC00667 knockdown significantly inhibited CRC cell growth and migration. YY1 transcription factor induced the upregulation of LINC00667 in CRC cells by transcriptionally activating LINC00667. In addition, miR-449b-5p could interact with LINC00667 in CRC cells. Intriguingly, miR-449b-5p directly targeted to YY1, thus inhibiting YY1 expression. YY1 recovered the CRC cell functions impai...Continue Reading

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Citations

May 5, 2021·Journal of Cellular and Molecular Medicine·Yangxue YinRong Zhou

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Methods Mentioned

BETA
surgical resection
PCR
Assay
transfection
immunoprecipitation
ChIP

Software Mentioned

GraphPad Prism
JASPAR
ALGGEN
DIANA
TargetScan
Cluster

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