LINC01272 Suppressed Cell Multiplication and Induced Apoptosis Via Regulating MiR-7-5p/CRLS1 Axis in Lung Cancer.

Journal of Microbiology and Biotechnology
Xuan MaXia Gao

Abstract

LINC01272 is a long non-coding RNA (lncRNA) that has been considered as a biomarker for many diseases including lung squamous cell carcinoma. Here, we investigated the function and mechanism of LINC01272 on lung cancer (LC). The differential expression of LINC01272 in LC and normal samples was analyzed by GEPIA based on the data from TCGA-LUAD database, as survival prognosis was analyzed through Kaplan-Meier Plotter. LINC01272 overexpression plasmid and miR-7-5p mimic were transfected into A549 and PC-9 cells. LINC01272, miR-7-5p and cardiolipin synthase 1 (CRLS1) mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction. Cell viability was detected through MTT assay. Cell multiplication was evaluated by cell formation assay. Cell apoptosis was assessed through flow cytometry assay. Through bioinformatics, the target miRNA of LINC01272 and downstream genes of miR-7-5p were predicted. The targeting relationship was tested by dual luciferase reporter analysis. CRLS1, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax) and cleaved caspase-3 protein levels were detected through western blot. LINC01272 was downregulated in LC and low LINC01272 expression had poor prognosis. In A549 and PC-9 cells, L...Continue Reading

References

Jun 8, 1971·Biochimica Et Biophysica Acta·K Y HostetlerL L Van Deenen
Oct 10, 2001·Biochimica Et Biophysica Acta·M ZörnigG Evan
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Grant M Hatch
Aug 5, 2005·Oncogene·Sophie LaunayCarmen Garrido
May 6, 2006·Cell Death and Differentiation·C GarridoG Kroemer
Apr 14, 2007·Cell Death and Differentiation·M OttS Orrenius
Jul 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ahmad M KhalilJohn L Rinn
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Feb 18, 2012·Nature·Mitchell Guttman, John L Rinn
Aug 4, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenbo LiuXiaobin Jia
Mar 7, 2013·Genetics·Johnny T Y KungJeannie T Lee
Jun 5, 2013·Cold Spring Harbor Perspectives in Biology·Amanda B ParrishSally Kornbluth
May 23, 2015·Nature Reviews. Cancer·Shuibin Lin, Richard I Gregory
Aug 31, 2016·Lancet·Fred R HirschLuis Paz-Ares
Sep 9, 2017·Advances in Cancer Research·Alessandra Drusco, Carlo M Croce
May 12, 2018·FEBS Letters·Igor Ulitsky
Dec 13, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·John HungAndrew H Baker
Jan 9, 2020·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Mar 21, 2020·International Journal of Environmental Research and Public Health·Kuang-Hsi ChangYi-Chao Hsu
May 6, 2020·OncoTargets and Therapy·Xue LengFangzhou Song

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis