LINC01287/miR-298/STAT3 feedback loop regulates growth and the epithelial-to-mesenchymal transition phenotype in hepatocellular carcinoma cells

Journal of Experimental & Clinical Cancer Research : CR
Yichao MoSiyao Che

Abstract

The long non-coding RNAs (lncRNAs) have participated in the promotion of hepatocellular carcinoma (HCC) initiation and progression. Nevertheless, the biological role and underlying mechanism of LINC01287 in HCC has never been reported. The TGCA database was used to explore the abnormal expression of lncRNAs in HCC. Real-time PCR and in situ hybridization assays were used to examine the expression of LINC01287 in HCC tissues. The clinicopathological characteristics of HCC patients in relation to LINC01287 expression were then analyzed. Infection of cells with the si-LINC01287 lentiviral vector was performed to down-regulate LINC01287 expression in HCC cells. MTT and colony formation assays were performed to examine cell growth ability, and FACS analysis was performed to examine the cell cycle and apoptosis. A Boyden assay was used to examine HCC cell invasion ability, and RNA immunoprecipitation tested the interaction between LINC01287 and miR-298. A luciferase reporter assay was used to examine whether STAT3 was a direct target of miR-298, and chromatin immunoprecipitation (ChIP) was used to examine the potential binding of c-jun to the miR-298 promoter. We revealed that the expression of LINC01287 was increased in HCC cell lin...Continue Reading

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Citations

Sep 1, 2019·In Vitro Cellular & Developmental Biology. Animal·Tao YeDongxin Tang
Nov 24, 2019·Journal of Cancer Research and Clinical Oncology·Rui ZhangShi-Yao Chen
May 22, 2020·Journal of Cellular and Molecular Medicine·Yi-Lin HuWan-Jiang Xue
Jun 2, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Chakrabhavi Dhananjaya MohanKanchugarakoppal S Rangappa

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Methods Mentioned

BETA
surgical resection
transfections
PCR
flow cytometry
immunoprecipitation
transfection
xenografts
RIP
xenograft

Software Mentioned

Targetscan
SPSS
Graph Pad Prism
PROMO
circlncRNAnet
UCSC

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