LincK contributes to breast tumorigenesis by promoting proliferation and epithelial-to-mesenchymal transition

Journal of Hematology & Oncology
Jing LiRobert Chunhua Zhao

Abstract

Increasing evidence has demonstrated that mesenchymal stem cells (MSCs) play a role in the construction of tumor microenvironments. Co-culture between tumor cells and MSCs provides an easy and useful platform for mimicking tumor microenvironments and identifying the important members involved in tumor progress. The long non-coding RNAs (lncRNAs) have been shown to regulate different tumorigenic processes. In this study, we aimed to examine functional lncRNA deregulations associated with breast cancer malignancy instigated by MSC-MCF-7 co-culture. The microarrays were used to profile the expression changes of lncRNAs in MCF-7 cells during epithelial-mesenchymal transition (EMT) induced by co-culture with MSCs. We found that an intergenic lncRNA KB-1732A1.1 (termed LincK, partly overlapped with GASL1) was significantly elevated. To investigate the biological function of LincK, the expression of EMT markers, cell migration, invasion, proliferation, and colony formation were evaluated in vitro and xenograft assay in nude mice were performed in vivo. Furthermore, we detected LincK expression in clinical samples using RNAscope® technology and verified aberrant expression of LincK in breast cancer data sets from The Cancer Genome Atla...Continue Reading

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Citations

Mar 20, 2020·Molecular Cancer·Francesca CrudeleStefano Volinia
Oct 10, 2020·Essays in Biochemistry·Jianjun Luo, Runsheng Chen
Jan 17, 2021·Pharmaceuticals·Laura Sofia CarvalhoJoão Nuno Moreira
Jan 29, 2021·Cancer Communications·Guangtao PanShenglan Yang
Apr 14, 2021·Cancer Letters·Jaganathan VenkateshPaola Marcato
May 9, 2021·Analytical Biochemistry·Bin WenKui Zhao
Jul 9, 2021·Data in Brief·Marie-Claire D WassonPaola Marcato

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Datasets Mentioned

BETA
GSE109007
GSE109008

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
transfection
PCR
Assay
immunoprecipitation
RIP
ELISA
pull down
xenograft
RNA-seq

Software Mentioned

LincK
GENCODE
Exiqon
GSEA
[UNK] RNAi Designer
BLOCK
ImageJ

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