Lincomycin-Induced Secondary Metabolism in Streptomyces lividans 66 with a Mutation in the Gene Encoding the RNA Polymerase Beta Subunit

Current Microbiology
Keiichiro MukaiTakeshi Hosaka

Abstract

Activating the genetic potential of Streptomyces strains to produce secondary metabolites can improve the production of useful biologically active compounds and facilitate the discovery of novel biologically active compounds. In this study, we found that Streptomyces lividans carrying the R440H mutation in rpoB, encoding the RNA polymerase beta subunit, grown in the presence of lincomycin at concentrations below the minimum inhibitory concentration (MIC) produced abundant amounts of actinorhodin and certain cryptic secondary metabolites despite culture conditions that restrict their production by the wild-type strain. The results indicate that lincomycin at concentrations below the MIC may strongly potentiate secondary metabolite production by Streptomyces strains carrying a specific rpoB mutation. In this study, we report an interesting phenomenon induced by combining the positive effects of certain rpoB mutations and concentration-dependent responses to lincomycin on secondary metabolism in S. lividans 66 and discuss the mechanisms and their applicability in exploring cryptic secondary metabolite production in streptomycetes.

References

Nov 30, 2004·Advances in Applied Microbiology·Kozo OchiKazuhiko Kurosawa
Apr 1, 2008·Journal of Bacteriology·Yasuo OhnishiSueharu Horinouchi
Nov 13, 2012·Applied Microbiology and Biotechnology·Kozo Ochi, Takeshi Hosaka
Mar 31, 2015·Applied and Environmental Microbiology·Yu ImaiTakeshi Hosaka
Sep 14, 2015·Journal of Industrial Microbiology & Biotechnology·Richard H Baltz
Sep 15, 2017·Molecular Microbiology·Stefanie Mak, Justin R Nodwell
Jan 27, 2018·Antonie van Leeuwenhoek·Misaki IshizukaTakeshi Hosaka
Oct 16, 2018·Frontiers in Microbiology·Hefa Mangzira KemungLearn-Han Lee
Jun 10, 2019·Journal of Industrial Microbiology & Biotechnology·Hailey A TommAvena C Ross

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