LincRNA-p21 acts as a mediator of ING1b-induced apoptosis

Cell Death & Disease
U M TranKarl Riabowol

Abstract

ING1b is a tumor suppressor that affects transcription, cell cycle control and apoptosis. ING1b is deregulated in disease, and its activity is closely linked to that of p53. In addition to regulating protein-coding genes, we found that ING1b also influences the expression of large intergenic non-coding RNAs (lincRNAs). In particular, lincRNA-p21 was significantly induced after DNA-damage stress or by ING1b overexpression. Furthermore, lincRNA-p21 expression in response to DNA damage was significantly attenuated in cells lacking ING1b. LincRNA-p21 is also a target of p53 and can trigger apoptosis in mouse cell models. We found that this function of lincRNA-p21 is conserved in human cell models. Moreover, ING1b and p53 could function independently to influence lincRNA-p21 expression. However, their effects become more additive under conditions of stress. In particular, ING1b regulates lincRNA-p21 levels by binding to its promoter and is required for induction of lincRNA-p21 by p53. The ability of ING1b to cause apoptosis is also impaired in the absence of lincRNA-p21. Surprisingly, deletion of the ING1b plant homeodomain, which allows it to bind histones and regulate chromatin structure, did not alter regulation of lincRNA-p21. O...Continue Reading

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Citations

Aug 14, 2015·International Journal of Molecular Sciences·Sai-Sai TangXing-Dong Xiong
Jul 22, 2015·Biochimica Et Biophysica Acta·Elena GrossiMaite Huarte
May 24, 2016·DNA and Cell Biology·Jingqiu LiZhaohui Gong
Aug 9, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Joan J CastellanoMariano Monzo
Dec 19, 2016·Wiley Interdisciplinary Reviews. RNA·Ritu Chaudhary, Ashish Lal
Jun 14, 2017·Journal of Cellular Biochemistry·Sadra Samavarchi TehraniBahman Yousefi
Nov 18, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Min LiXian-Ming Chen
Jun 9, 2017·Scientific Reports·Xiaolong TuJunfeng Zhang
Jan 20, 2018·Experimental & Molecular Medicine·Yue ZhangJun Tang
Jan 24, 2019·Cancer Letters·Marjorie GournayRémy Pedeux

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Methods Mentioned

BETA
immunoprecipitation
transfection
X-ray
transfections
PCR
ChIP

Software Mentioned

AxioVision

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis