PMID: 9650987Jul 3, 1998Paper

Line probe assay for detection of human immunodeficiency virus type 1 mutations conferring resistance to nucleoside inhibitors of reverse transcriptase: comparison with sequence analysis

Journal of Clinical Microbiology
D DescampsFrançoise Brun-Vézinet

Abstract

We compared the line probe assay (LiPA) to sequence analysis for the detection of mutations conferring resistance to nucleoside inhibitors of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT). Plasma samples from 40 patients who had received zidovudine, dideoxyinosine, and dideoxycytosine, alone or in combination, and who were enrolled in the ALTIS 2 clinical trial (lamivudine [3TC] plus stavudine) were tested at enrollment and at week 24. RT PCR products from plasma were used for LiPA, and DNA was used for sequence analysis. LiPA gave uninterpretable results for 8.5% of the analyzed codons corresponding to 63 samples, mainly for codons 41, 69, and 70. Several minor discrepancies between the two methods occurred, mainly due to the ability of LiPA to detect mixed populations while sequence analyses detect a single homogeneous population. LiPA is suitable for detecting mixed populations and easy to implement in clinical laboratories and might be useful for epidemiological surveys of primary HIV-1 resistance.

References

May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C López-GalíndezM Perucho
Sep 1, 1996·Journal of Clinical Microbiology·L StuyverG Maertens

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Citations

Jan 14, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·R CésaireA Cabié
Jun 22, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P R Harrigan, H C Côté
Jun 6, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·C de MendozaJ González-Lahoz

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