Lineage and host source are both correlated with levels of Shiga toxin 2 production by Escherichia coli O157:H7 strains.

Applied and Environmental Microbiology
Yongxiang ZhangVictor Gannon

Abstract

Escherichia coli O157:H7 strains fall into three major genetic lineages that differ in their distribution among humans and cattle. Several recent studies have reported differences in the expression of virulence factors between E. coli O157:H7 strains from these two host species. In this study, we wished to determine if important virulence-associated "mobile genetic elements" such as Shiga toxin 2 (Stx2)-encoding prophage are lineage restricted or are host source related and acquired independently of the pathogen genotype. DNA sequencing of the stx(2) flanking region from a lineage II (LII) strain, EC970520, revealed that the transcriptional activator gene Q in LI strain EDL933 (upstream of stx(2)) is replaced by a pphA (serine/threonine phosphatase) homologue and an altered Q gene in this and all other LII strains tested. In addition, nearly all LI strains carried stx(2), whereas all LII strains carried variant stx(2c) and 4 of 14 LI/II strains had copies of both stx(2) and variant stx(2c). Real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) demonstrated that LI and LI/II strains produce significantly more stx(2) mRNA and Stx2 than LII strains. However, among LI strains significantly more Stx2 is also produced ...Continue Reading

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Citations

Jan 1, 2013·Applied and Environmental Microbiology·Dongjin ParkCharles W Kaspar
Jul 18, 2012·BMC Genomics·Darren L SmithHeather E Allison
Mar 26, 2016·Frontiers in Microbiology·Mohamed Elhadidy, Avelino Álvarez-Ordóñez
Dec 7, 2014·Microbiology·Alejandra Krüger, Paula M A Lucchesi
Jul 30, 2015·Diagnostic Microbiology and Infectious Disease·Mohamed ElhadidyMarc Heyndrickx
Nov 8, 2013·BioMed Research International·Kevin J AllenLinda Hoang
Nov 28, 2014·Epidemiology and Infection·M M Elhadidy, W F Elkhatib
Mar 29, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yoshiki EtohTomoko Yamamoto
Jan 25, 2012·FEMS Microbiology Letters·Kjersti HaugumLin Thorstensen Brandal
Feb 6, 2020·Antimicrobial Agents and Chemotherapy·Sabrina MühlenPetra Dersch
May 5, 2017·Frontiers in Cellular and Infection Microbiology·Tungadri BoseSharmila S Mande

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