Jun 2, 2020

Lineage and Spatial Mapping of Glioblastoma-associated Immunity

BioRxiv : the Preprint Server for Biology
Vidhya Madapusi RaviD. H. Heiland

Abstract

The diversity of molecular states and cellular plasticity of immune cells within the glioblastoma (GBM) environment remain poorly investigated. Here, we performed deep transcriptional profiling of lymphoid and myeloid cell populations by scRNA-sequencing, and mapped potential cellular interactions and cytokine responses that lead to the dysfunctional and exhausted phenotype of T cells. We identified the Interleukin 10 (IL-10) response during T cell activation as leading to a dysfunctional state in T cells. By the use of a novel method, the nearest functionally connected neighbor (NFCN), an in-silico model to explore cell-cell interactions, the dysfunctional/exhausted phenotype was found to be driven by a subset of myeloid cells defined by high expression of HMOX1. By using spatial transcriptomic RNA-sequencing, we identified a correlation between T cell exhaustion and colocalized mesenchymal gene expression also found that HMOX1 expressing myeloid cells occupied regions marked by T cell exhaustion. Using a human neocortical slice model with myeloid cell depletion we confirmed the functional interaction of myeloid and lymphoid cells, leading to the dysfunctional state of T cells. A comprehensive understanding of cellular states ...Continue Reading

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Mentioned in this Paper

T-Cell Activation
Mesenchyma
Protein Expression
Proximal
Exhaustion
Myeloid Cells
HMOX1 gene
Heme Oxygenase-1
Single Linkage Cluster Analysis
Immune Effector Cell

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