Lineage-Specific Wnt Reporter Elucidates Mesenchymal Wnt Signaling during Bone Repair

The American Journal of Pathology
Leslie ChangAaron W James

Abstract

β-Catenin-dependent Wnt signaling controls numerous aspects of skeletal development and postnatal bone repair. Currently available transgenic Wnt reporter mice allow for visualization of global canonical Wnt signaling activity within skeletal tissues, without delineation of cell type. This is particularly important in a bone repair context, in which the inflammatory phase can obscure the visualization of mesenchymal cell types of interest. To tackle the issue of tissue-specific Wnt signaling, we have generated and characterized a transgenic mouse strain [termed paired related homeobox 1 (Prx1)-Wnt-green fluorescent protein (GFP), by crossing a previously validated Prx1-Cre strain with a nuclear fluorescent reporter driven by T-cell factor/lymphoid enhancer factor activity (Rosa26-Tcf/Lef-LSL-H2B-GFP)]. Prx1-Wnt-GFP animals were subject to three models of long bone and membranous bone repair (displaced forelimb fracture, tibial cortical defect, and frontal bone defect). Results showed that, irrespective of bone type, locoregional mesenchymal cell activation of Wnt signaling occurs in a defined temporospatial pattern among Prx1-Wnt-GFP mice. In summary, Prx1-Wnt-GFP reporter animals allow for improved visualization, spatial discr...Continue Reading

References

Jun 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T KonT A Einhorn
Jan 26, 2002·Molecular and Cellular Biology·Barbara LustigJürgen Behrens
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Silvia MarettoStefano Piccolo
Jun 9, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Julie R HensJohn J Wysolmerski
Jun 23, 2009·Biochemical and Biophysical Research Communications·Aya KawanamiShunichi Murakami
May 28, 2011·Tissue Engineering. Part a·Xinli ZhangChia Soo
Oct 2, 2012·Cold Spring Harbor Perspectives in Biology·Ken M Cadigan, Marian L Waterman
Mar 12, 2013·Journal of Bone and Mineral Metabolism·Hiroki MuraoHaruhiko Akiyama
Aug 29, 2014·Arthritis Research & Therapy·Esmeralda N Blaney DavidsonPeter M van der Kraan
Mar 8, 2016·Bone·Florence LoiStuart B Goodman
Oct 30, 2016·Arthritis & Rheumatology·Yuki TaniguchiTaku Saito
Aug 5, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jochen KronerAnita Ignatius

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