The vertebrate body forms by continuous generation of new tissue from progenitors at the posterior end of the embryo. The study of these axial progenitors has proved to be challenging in vivo largely because of the lack of unique molecular markers to identify them. Here, we elucidate the expression pattern of the transcription factor Nkx1-2 in the mouse embryo and show that it identifies axial progenitors throughout body axis elongation, including neuromesodermal progenitors and early neural and mesodermal progenitors. We create a tamoxifen-inducible Nkx1-2CreERT2 transgenic mouse and exploit the conditional nature of this line to uncover the lineage contributions of Nkx1-2-expressing cells at specific stages. We show that early Nkx1-2-expressing epiblast cells contribute to all three germ layers, mostly neuroectoderm and mesoderm, excluding notochord. Our data are consistent with the presence of some self-renewing axial progenitors that continue to generate neural and mesoderm tissues from the tail bud. This study identifies Nkx1-2-expressing cells as the source of most trunk and tail tissues in the mouse and provides a useful tool to genetically label and manipulate axial progenitors in vivo.
The chicken homeo box genes CHox1 and CHox3: cloning, sequencing and expression during embryogenesis
Live imaging and genetic analysis of mouse notochord formation reveals regional morphogenetic mechanisms
Localised axial progenitor cell populations in the avian tail bud are not committed to a posterior Hox identity
Transthyretin mouse transgenes direct RFP expression or Cre-mediated recombination throughout the visceral endoderm
Redefining the progression of lineage segregations during mammalian embryogenesis by clonal analysis
Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells
Loss of FGF-dependent mesoderm identity and rise of endogenous retinoid signalling determine cessation of body axis elongation
Integration of signals along orthogonal axes of the vertebrate neural tube controls progenitor competence and increases cell diversity
In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity
Wnt/β-catenin and FGF signalling direct the specification and maintenance of a neuromesodermal axial progenitor in ensembles of mouse embryonic stem cells
Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation
A Gene Regulatory Network Balances Neural and Mesoderm Specification during Vertebrate Trunk Development
Antagonistic Activities of Sox2 and Brachyury Control the Fate Choice of Neuro-Mesodermal Progenitors
Neural differentiation, selection and transcriptomic profiling of human neuromesodermal progenitor-like cells in vitro.
Myc activity is required for maintenance of the neuromesodermal progenitor signalling network and for segmentation clock gene oscillations in mouse.
A dorsal-ventral gradient of Wnt3a/β-catenin signals controls mouse hindgut extension and colon formation.
Sall4 regulates neuromesodermal progenitors and their descendants during body elongation in mouse embryos
The transcription factor NKX1-2 promotes adipogenesis and may contribute to a balance between adipocyte and osteoblast differentiation.
Dynamic extrinsic pacing of the HOX clock in human axial progenitors controls motor neuron subtype specification.
Spatiotemporal contribution of neuromesodermal progenitor-derived neural cells in the elongation of developing mouse spinal cord.
Adult Stem Cells
Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.