Lineage tracing of axial progenitors using Nkx1-2CreERT2 mice defines their trunk and tail contributions.

Aida Rodrigo AlborsK G Storey


The vertebrate body forms by continuous generation of new tissue from progenitors at the posterior end of the embryo. The study of these axial progenitors has proved to be challenging in vivo largely because of the lack of unique molecular markers to identify them. Here, we elucidate the expression pattern of the transcription factor Nkx1-2 in the mouse embryo and show that it identifies axial progenitors throughout body axis elongation, including neuromesodermal progenitors and early neural and mesodermal progenitors. We create a tamoxifen-inducible Nkx1-2CreERT2 transgenic mouse and exploit the conditional nature of this line to uncover the lineage contributions of Nkx1-2-expressing cells at specific stages. We show that early Nkx1-2-expressing epiblast cells contribute to all three germ layers, mostly neuroectoderm and mesoderm, excluding notochord. Our data are consistent with the presence of some self-renewing axial progenitors that continue to generate neural and mesoderm tissues from the tail bud. This study identifies Nkx1-2-expressing cells as the source of most trunk and tail tissues in the mouse and provides a useful tool to genetically label and manipulate axial progenitors in vivo.


Apr 1, 1984·The American Journal of Anatomy·G C Schoenwolf
May 1, 1995·Mechanisms of Development·F R SchubertP Gruss
Nov 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K SulikG C Schoenwolf
Jan 1, 1993·Methods in Enzymology·D G Wilkinson, M A Nieto
Mar 1, 1996·Mechanisms of Development·V Wilson, R S Beddington
Apr 12, 2001·BMC Developmental Biology·S SrinivasF Costantini
Sep 17, 2002·Genes & Development·Jane BrennanElizabeth J Robertson
Jun 9, 2004·Gene Expression Patterns : GEP·Young-Ki BaeMasahiko Hibi
Aug 26, 2004·Mechanisms of Development·Laura Anne Lowery, Hazel Sive
Sep 6, 2005·Development·Mariana Delfino-MachínKate G Storey
Jul 6, 2007·Development·Noemí Cambray, Valerie Wilson
Apr 28, 2009·Development·Valerie WilsonKate G Storey
May 6, 2009·Genesis : the Journal of Genetics and Development·Gloria S Kwon, Anna-Katerina Hadjantonakis
Sep 18, 2009·Developmental Cell·Elena TzouanacouJean-François Nicolas
Oct 21, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Alisa S W ShumHenk Roelink
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jun 15, 2013·Developmental Cell·Arnon Dias JurbergMoisés Mallo
Aug 6, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Roel NeijtsJacqueline Deschamps
Aug 26, 2014·Developmental Biology·Arnon Dias JurbergMoisés Mallo
Nov 6, 2014·Development·Ravindra B ChalamalasettyTerry P Yamaguchi
Nov 11, 2014·Wiley Interdisciplinary Reviews. Developmental Biology·Caroline W Beck
Feb 12, 2015·Development·Anestis TsakiridisValerie Wilson
Sep 4, 2015·Development·Domingos HenriqueKate G Storey
Sep 25, 2015·F1000Research·Anestis Tsakiridis, Valerie Wilson
Mar 13, 2016·Current Topics in Developmental Biology·David Kimelman
Jul 28, 2016·Developmental Cell·Rita AiresMoisés Mallo
Feb 14, 2017·Developmental Biology·Ben Steventon, Alfonso Martinez Arias
Feb 16, 2017·Development·Evanthia NikolopoulouAndrew J Copp

❮ Previous
Next ❯


Dec 4, 2019·Birth Defects Research·Dawn SaveryGabriel L Galea
Oct 28, 2020·Development·Manuel RochaVictoria E Prince
Feb 18, 2021·Development·Filip J WymeerschAnestis Tsakiridis
Feb 21, 2021·Nature Communications·Gabriel L GaleaAndrew J Copp
Apr 26, 2021·Journal of Korean Neurosurgical Society·Mohammed R ShakerWoong Sun

❮ Previous
Next ❯

Methods Mentioned


Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.