Linear chromosome-generating system of Agrobacterium tumefaciens C58: protelomerase generates and protects hairpin ends.

The Journal of Biological Chemistry
Wai Mun HuangSherwood Casjens

Abstract

Agrobacterium tumefaciens C58, the pathogenic bacteria that causes crown gall disease in plants, harbors one circular and one linear chromosome and two circular plasmids. The telomeres of its unusual linear chromosome are covalently closed hairpins. The circular and linear chromosomes co-segregate and are stably maintained in the organism. We have determined the sequence of the two ends of the linear chromosome thus completing the previously published genome sequence of A. tumefaciens C58. We found that the telomeres carry nearly identical 25-bp sequences at the hairpin ends that are related by dyad symmetry. We further showed that its Atu2523 gene encodes a protelomerase (resolvase) and that the purified enzyme can generate the linear chromosomal closed hairpin ends in a sequence-specific manner. Agrobacterium protelomerase, whose presence is apparently limited to biovar 1 strains, acts via a cleavage-and-religation mechanism by making a pair of transient staggered nicks invariably at 6-bp spacing as the reaction intermediate. The enzyme can be significantly shortened at both the N and C termini and still maintain its enzymatic activity. Although the full-length enzyme can uniquely bind to its product telomeres, the N-terminal...Continue Reading

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Citations

Sep 18, 2013·Cellular and Molecular Life Sciences : CMLS·Nick FulcherKarel Riha
May 27, 2015·Nucleic Acids Research·Danica LucyshynKerri Kobryn
May 11, 2016·Chemical Reviews·Gretchen MeinkeFrank Buchholz
Jul 1, 2017·Applied Microbiology and Biotechnology·Lucía Feijoo-SiotaTomás G Villa
Feb 5, 2021·PloS One·Siobhan L McGrathKerri Kobryn
Jun 25, 2015·Microbiology Spectrum·Kerri Kobryn, George Chaconas
Jun 25, 2015·Microbiology Spectrum·Nikolai V Ravin

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