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Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system

ACS Synthetic Biology

Jun 20, 2014

Zachary Z. SunRichard M. Murray

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Abstract

Accelerating the pace of synthetic biology experiments requires new approaches for rapid prototyping of circuits from individual DNA regulatory elements. However, current testing standards require days to weeks due to cloning and in vivo transformation. In this work, we first characteri...read more

Mentioned in this Paper

Gene Expression
Early Promoters, Genetic
Sequence Determinations, DNA
Cell-Free System
Promoter
Regulatory Sequences, Nucleic Acid
In Vitro [Publication Type]
Plasmids
Escherichia coli
Exonuclease
2
230
Paper Details
References
  • References20
  • Citations33
12
  • References20
  • Citations33
1234

Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system

ACS Synthetic Biology

Jun 20, 2014

Zachary Z. SunRichard M. Murray

PMID: 24303785

DOI: 10.1021/sb400131a

Abstract

Accelerating the pace of synthetic biology experiments requires new approaches for rapid prototyping of circuits from individual DNA regulatory elements. However, current testing standards require days to weeks due to cloning and in vivo transformation. In this work, we first characteri...read more

Mentioned in this Paper

Gene Expression
Early Promoters, Genetic
Sequence Determinations, DNA
Cell-Free System
Promoter
2
230

Related Papers

Paper Details
References
  • References20
  • Citations33
12
  • References20
  • Citations33
1234

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