PMID: 8956081Nov 1, 1996Paper

Linear epitopes of sperm whale myoglobin identified by polyclonal antibody screening of random peptide library

International Journal of Peptide and Protein Research
Z J YaoM C Chung

Abstract

Distinct enhancement of antibody-specific clones was apparent during the screening against random peptide libraries with antigen-specific polyclonal antibodies. Several sequence motifs obtained from these screenings were homologous with the primary sequence of myoglobin. Two of these motifs have been confirmed as antigenic determinants by competitive inhibition tests using eight-branched synthetic peptides. One of the peptides has a sequence that corresponds to amino acid residues 42-50, KFDRFKHLK, of the myoglobin sequence. This is a new epitope of myoglobin that is reported for the first time. The epitope is located precisely in the "turn' or "loop' region between helices C and D of the crystal structure of myoglobin. The second antibody binding site has a sequence of DIAAKYKELGYQG, and this is located between residues 141-153, which is the C-terminus of myoglobin. This epitope encompassed two linear epitopes of myoglobin, amino acid residues 145-151 and 147-153, that have been reported earlier based on immunochemical characterisation of cleavage fragments of the protein. These results clearly indicate that epitope mapping using polyclonal antibodies against random peptide libraries can identify new epitopes precisely, as wel...Continue Reading

References

Jul 27, 1990·Science·J K Scott, G P Smith
Jun 1, 1991·International Journal of Peptide and Protein Research·A FurkaG Dibó
Jun 26, 1987·Journal of Immunological Methods·J D BeattyW G Vlahos
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B A Springer, S G Sligar
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J P Tam
Jan 1, 1984·Annual Review of Immunology·D C BenjaminE M Prager
Jan 1, 1983·Biopolymers·S J Leach
Mar 25, 1981·Journal of Molecular Biology·T F Smith, M S Waterman
Apr 1, 1993·Current Opinion in Immunology·M E Goldberg, L Djavadi-Ohaniance
Mar 1, 1965·The Biochemical Journal·M J Crumpton, J M Wilkinson

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Citations

Aug 27, 1998·Molecular Biotechnology·F al-ObeidiT K Sawyer
Aug 26, 2020·Journal of the American Society for Mass Spectrometry·Delia MihocMichael Przybylski

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