Linear models of ovine IgG1 and IgG2 subclasses and predicted pepsin cleavage sites

Journal of Pharmaceutical and Biomedical Analysis
Russell G A Jones, Angela Martino

Abstract

Highly purified specific Fab antibody fragments derived from sheep have a long history of therapeutic use as safe and effective emergency medicines. In more recent years simple low-cost methods have been developed, which take advantage of the ability of pepsin under optimally controlled conditions to preferentially digest ovine IgG within the Fc region to produce F(ab')2 and easy to remove low MW Fc sub-fragments. Despite these developments no information is currently available on the pepsin digestion of ovine IgG at the amino acid level hindering the development of improved F(ab')2 processing methods. To gain knowledge of the fragments properties we have constructed linear models of ovine IgG1 and IgG2 subclasses, starting from the gamma-1 and gamma-2 chain amino acid sequences, which also incorporate the inter- and intra-chain disulphide bonds. Any potential pepsin cleavage site was initially predicted in silico, then high probability points identified for each of the molecules and mapped onto the individual models. A theoretical order of digestion was subsequently constructed, which appeared to agree with the experimental data, suggesting an accurate prediction model for ovine IgG1 and IgG2 subclasses. These findings lay the...Continue Reading

References

Jan 1, 1978·International Archives of Allergy and Applied Immunology·D R StanworthD M Moran
Feb 1, 1978·Annals of the Rheumatic Diseases·F Shakib, D R Stanworth
Dec 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J HamakoK Titani
Jan 30, 2009·Journal of Immunological Methods·Russell G A JonesDorothea Sesardic
Jan 21, 2015·Critical Reviews in Biotechnology·Russell G A Jones, Angela Martino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.