Linezolid is a specific inhibitor of 50S ribosomal subunit formation in Staphylococcus aureus cells

Current Microbiology
W S Champney, Mindy Miller

Abstract

Linezolid is an oxazolidinone compound that has been shown to have impressive antimicrobial activity against a number of Gram-positive bacteria. It inhibits an initiation step of protein synthesis, and its binding site has been shown to be on the 50S ribosomal subunit. Linezolid was tested to see whether would interfere with the formation of the 50S subunit in Staphylococcus aureus cells, since a number of other 50S-specific antibiotics have this second inhibitory function. Linezolid inhibited protein synthesis in S. aureus cells with an IC50 of 0.3 microg/ml. A concentration-dependent decline in cell number with an increase in generation time was found. Pulse-chase labeling studies revealed a specific inhibitory effect on 50S particle formation, with no effect on 30S subunit assembly. The compound inhibited 50S synthesis with an IC50 of 0.6 microg/ ml, indicating an equivalent effect on translation and particle assembly. A postantibiotic effect of 1 h was found when cells were initially treated with the drug at 2 microg/ ml. 50S particle numbers recovered more rapidly than translational capacity, consistent with the increase in viable cell numbers. The inhibitory activities of this novel antimicrobial agent in cells are discus...Continue Reading

Citations

Feb 5, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·George M Eliopoulos
Jun 15, 2007·Antimicrobial Agents and Chemotherapy·W Scott Champney, Ward K Rodgers
Jun 18, 2011·The Medical Clinics of North America·Luke F ChenKeith S Kaye
Nov 17, 2009·Infectious Disease Clinics of North America·Luke F ChenKeith S Kaye
Sep 11, 2004·Mayo Clinic Proceedings·Raymund R RazonableJames M Steckelberg
Mar 24, 2006·Molecular Microbiology·Tanel Tenson, Alexander Mankin
Feb 4, 2003·Expert Opinion on Pharmacotherapy·Milind Khare, Deirbhile Keady
Oct 6, 2009·International Journal of Medical Microbiology : IJMM·Nadine McCallumMaria M Senn
Mar 29, 2005·The Lancet Infectious Diseases·Robert Ew Hancock
Feb 12, 2005·The Journal of Antimicrobial Chemotherapy·Tony WhitehouseA Peter R Wilson
Jan 17, 2020·The Journal of Antimicrobial Chemotherapy·W Scott Champney
Apr 12, 2003·The Journal of Biological Chemistry·Jerry R ColcaAlexander S Mankin
May 6, 2005·Pediatric Emergency Care·Samir S Shah

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.