Linezolid resistance in Enterococcus faecium and Enterococcus faecalis from hospitalized patients in Ireland: high prevalence of the MDR genes optrA and poxtA in isolates with diverse genetic backgrounds.

The Journal of Antimicrobial Chemotherapy
Sarah A EganDavid C Coleman

Abstract

To investigate the prevalence of the optrA, poxtA and cfr linezolid resistance genes in linezolid-resistant enterococci from Irish hospitals and to characterize associated plasmids. One hundred and fifty-four linezolid-resistant isolates recovered in 14 hospitals between June 2016 and August 2019 were screened for resistance genes by PCR. All isolates harbouring resistance genes, and 20 without, underwent Illumina MiSeq WGS. Isolate relatedness was assessed using enterococcal whole-genome MLST. MinION sequencing (Oxford Nanopore) and hybrid assembly were used to resolve genetic environments/plasmids surrounding resistance genes. optrA and/or poxtA were identified in 35/154 (22.7%) isolates, the highest prevalence reported to date. Fifteen isolates with diverse STs harboured optrA only; one Enterococcus faecium isolate harboured optrA (chromosome) and poxtA (plasmid). Seven Enterococcus faecalis and one E. faecium harboured optrA on a 36 331 bp plasmid with 100% identity to the previously described optrA-encoding conjugative plasmid pE349. Variations around optrA were also observed, with optrA located on plasmids in five isolates and within the chromosome in three isolates. Nine E. faecium and 10 E. faecalis harboured poxtA, fla...Continue Reading

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Citations

Jan 15, 2021·Diagnostic Microbiology and Infectious Disease·Franziska LayerGuido Werner
Feb 2, 2021·International Journal of Medical Microbiology : IJMM·Flaminia OlearoHolger Rohde
Feb 21, 2021·Applied and Environmental Microbiology·Simona FioritiEleonora Giovanetti
Apr 26, 2021·The Journal of Antimicrobial Chemotherapy·Loren DejoiesVincent Cattoir
May 10, 2021·Letters in Applied Microbiology·L D R Dos SantosE G Stehling
Jun 3, 2021·Clinical Microbiology Reviews·Stefan SchwarzYang Wang
Jun 8, 2021·Antimicrobial Agents and Chemotherapy·Ilaria BaccaniGian Maria Rossolini
Sep 11, 2021·Irish Journal of Medical Science·Kornelia Maria DembickaColum P Dunne

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Datasets Mentioned

BETA
MG707078.1
MN831418
MN831419

Methods Mentioned

BETA
PCR
Genotyping

Software Mentioned

Unicycler
SAMtools
wgMLST
BEDTools
cgMLST
SPAdes
BioNumerics

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.