Linkage mapping and genome-wide association reveal candidate genes conferring thermotolerance of seed-set in maize

Journal of Experimental Botany
Jingyang GaoJianyu Wu

Abstract

It is predicted that high-temperature stress will increasingly affect crop yields worldwide as a result of climate change. In order to determine the genetic basis of thermotolerance of seed-set in maize under field conditions, we performed mapping of quantitative trait loci (QTLs) in a recombinant inbred line (RIL) population using a collection of 8329 specifically developed high-density single-nucleotide polymorphism (SNP) markers, combined with a genome-wide association study (GWAS) of 261 diverse maize lines using 259 973 SNPs. In total, four QTLs and 17 genes associated with 42 SNPs related to thermotolerance of seed-set were identified. Among them, four candidate genes were found in both linkage mapping and GWAS. Thermotolerance of seed-set was increased significantly in near-isogenic lines (NILs) that incorporated the four candidate genes in a susceptible parent background. The expression profiles of two of the four genes showed that they were induced by high temperatures in the maize tassel in a tolerant parent background. Our results indicate that thermotolerance of maize seed-set is regulated by multiple genes each of which has minor effects, with calcium signaling playing a central role. The genes identified may be ex...Continue Reading

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Citations

Nov 21, 2019·Physiologia Plantarum·Yuanyuan WangShoubing Huang
Dec 12, 2019·F1000Research·Baljeet SinghUmesh Goutam
Feb 10, 2021·Plants·Muhammad Ahmed WaqasMuhammad Farooq
Dec 5, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·David Sewordor GaikpaThomas Miedaner
Apr 15, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jacob I MarshDavid Edwards
May 11, 2021·3 Biotech·Kumari ShikhaB Sinha

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